Inhibition of histamine release from human mast cells by natural chymase inhibitors

• Published in: Acta pharmacologica Sinica Vol. 25; no. 6; pp. 822 - 826
• Main Authors: He, Shao-Heng, Xie, Hua, Zhang, Xiao-Jun, Wang, Xian-Jie
• Format: Journal Article
• Language: English
• Published: United States 01.06.2004
• Subjects: alpha 1-Antitrypsin - pharmacology; Antibodies, Anti-Idiotypic - pharmacology; Calcimycin - pharmacology; Chymases; Histamine Release - drug effects; Humans; Ionophores - pharmacology; Lung - cytology; Mast Cells - metabolism; Palatine Tonsil - cytology; Proteinase Inhibitory Proteins, Secretory; Proteins - pharmacology; Secretory Leukocyte Peptidase Inhibitor; Serine Endopeptidases - pharmacology; Serine Proteinase Inhibitors - pharmacology; Skin - cytology; α1-抗胰蛋白酶; 人类免疫细胞; 免疫球蛋白E; 抑制作用; 敏感症; 糜蛋白酶; 组胺释放; 钙离子
• ISSN: 1671-4083; 1745-7254

AIM: To investigate the ability of natural chymase inhibitors to modulate histamine release from human mast cells.METHODS: Enzymatically dispersed cells from human lung, tonsil, and skin were challenged with anti-IgE or calcium ionophore A23187 in the absence or presence of the natural chymase inhibitors secretory leukocyte protease inhibitor (SLPI) and α1-antitrypsin, then histamine release was determined. RESULTS: IgE-dependent histamine release from lung, tonsil, and skin mast cells were inhibited by up to 70 %, 61%, and 62 %, respectively following incubation with α1-antitrypsin (5000 nmol/L). SLPI 5000 nmol/L was also able to inhibit anti-IgE-dependent histamine released from lung, tonsil and skin mast cells by up to approximately 72 %, 67 %, and 58 %,respectively. While neither α1-antitrypsin nor SLPI by themselves altered histamine release from lung, tonsil and skin mast cells, they were able to inhibit calcium ionophore-induced histamine release from lung and tonsil mast cells. CONCLUSION: Both α1-antitrypsin and SLPI could potently inhibit IgE-dependent and calcium ionophoreinduced histamine release from dispersed human lung, tonsil, and skin mast cells in a concentration-dependent manner, which suggested that they were likely to play a protective role in mast cell associated diseases including allergy.