Phosphate solubilization by Antarctic yeasts isolated from lichens

• Published in: Archives of microbiology Vol. 204; no. 12; p. 698
• Main Authors: da Silva, Averlane Vieira, da Silva, Mayanne Karla, de Oliveira, Adeildo Junior, Silva, José Vieira, Paulino, Sivaldo Soares, de Queiroz, Aline Cavalcanti, Leite, Jakson, França, Paulo Henrique Barcellos, Putzke, Jair, Montone, Rosalinda, de Oliveira, Valéria Maia, dos Santos, Viviane Piccin, Rosa, Luiz Henrique, Duarte, Alysson Wagner Fernandes
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2022; Springer Nature B.V
• Subjects: Acetic acid; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Ecology; Lichens; Life Sciences; Liquid chromatography; Microbial Ecology; Microbiology; Microorganisms; Organic acids; Original Paper; pH effects; Phosphate; Phosphates; Proteomics; Screening; Solubilization; Vishniacozyma; Yeast; Yeasts; Antarctica; Phosphate-solubilizing microorganisms; Tartaric acid; Organic acids
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-03310-0

Antarctica has a great diversity of microorganisms with biotechnological potential but is not very well Known about yeasts with phosphate solubilization activity. Thus, the aim of this study was to evaluate the ability of yeasts from Antarctica lichens to solubilize phosphate in vitro. In the screening, 147 yeasts were tested and 43 (29%) showed P solubilization in solid NBRIP medium at 15.0 °C, with a higher prevalence of positive genera Vishniacozyma , followed by Cystobasidium . Most of the positive yeasts were isolated from Usnea auratiacoatra , followed by Polycauliona regalis and Lecania brialmontii . Two strains with better activity after screening were selected for the solubilization in the liquid medium, Vishniacozyma victoriae 2.L15 and A.L6 (unidentified). Vishniacozyma victoriae 2.L15 exhibiting activities at 25.0 °C (29.91 mg/L of phosphate and pH 6.85) and at 30.0 °C (619.04 mg/L of phosphate and pH 3.73) and A.L6 strain at 25.0 °C (25.05 mg/L of phosphate and pH 6.69) and at 30.0 °C (31.25 mg/L of phosphate and pH 6.47). Of eight organic acids tested by HPLC, tartaric and acetic acids were detected during phosphate solubilization, with greater release in the period of 144 (2.13 mg/L) and 72 (13.72 mg/L) hours, respectively. Future studies to elucidate the presence of functional genes for P metabolism in lichens, as well as studies in the field of proteomics for the discovery of yeast proteins related to P solubilization are needed. Thus, the high prevalence of lichen-associated yeast communities probably contributed to the high frequency of phosphate-solubilizing isolates in this study. Graphical abstract