Labeled sandwich-type electrochemical immunosensor based on Ti3C2Tx/AuNP and Ti3C2Tx/HKUST-1/TB composites for early liver cancer detection

A novel sandwich-type electrochemical immunosensor for the detection of the liver cancer marker alpha-fetoprotein (AFP) in human serum is proposed. The two-dimensional MXene material Ti 3 C 2 T x was first prepared using etching and ultrasonic stripping, and then Ti 3 C 2 T x was used to reduce chlo...

Full description

Saved in:
Bibliographic Details
Published inMikrochimica acta (1966) Vol. 191; no. 9; p. 565
Main Authors Wu, Haotian, Zhang, Gang, Yang, Xiaozhan
Format Journal Article
LanguageEnglish
Published Vienna Springer Vienna 01.09.2024
Springer Nature B.V
Subjects
Analytical Chemistry
Antibodies
Binding sites
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chloroauric acid
Composite materials
Glassy carbon
Immunosensors
Liver cancer
Metal-organic frameworks
Microengineering
Nanochemistry
Nanotechnology
Original Paper
Toluidine blue
Toluidine blue
Gold nanoparticles
MXene
Electrochemical immunosensor
Modified glassy carbon electrode
Differential pulse voltammetry
Metal–organic frameworks
Online AccessGet full text

Cover

More Information
Summary:A novel sandwich-type electrochemical immunosensor for the detection of the liver cancer marker alpha-fetoprotein (AFP) in human serum is proposed. The two-dimensional MXene material Ti 3 C 2 T x was first prepared using etching and ultrasonic stripping, and then Ti 3 C 2 T x was used to reduce chloroauric acid to form Ti 3 C 2 T x /AuNP composites which were modified on the surface of the glassy carbon electrodes to form probe-type sensors. The Ti 3 C 2 T x /AuNPs provide a large number of binding sites for the AFP capture antibody (Ab1) and increase the electrochemical reaction active site. The Ti 3 C 2 T x /copper metal–organic frameworks HKUST-1 composite was also prepared by solvothermal method and combined with toluidine blue (TB) and AFP detection antibody (Ab2) to form a labeled sandwich-type electrochemical immunosensor. The sensor achieved trace detection of AFP from 0.1 to 100 ng/mL with a detection limit of 0.073 pg/mL and possesses good selectivity, stability, and reproducibility. The sensor performs well in clinical samples and has good potential for clinical applications. Graphical Abstract
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0026-3672
1436-5073
1436-5073
DOI:10.1007/s00604-024-06618-4