A COMPARATIVE STUDY ON THE PERFORMANCE OF TWO COMMERCIAL SEROLOGICAL DENGUE KITS

The performance of a commercial rapid immunochromatographic dengue NS1, IgG/IgM assay device was evaluated against an in-place dengue IgM-capture ELISA in the KPC Medical College & Hospital, Jadavpur. Of the 932 serum samples from patients with clinical diagnosis of acute dengue illness, 542, 23...

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Bibliographic Details
Published inJournal of evolution of medical and dental sciences Vol. 4; no. 10; pp. 1659 - 1665
Main Authors Bibhas, Saha Dalal, Swagnik, Roy, Saurabh, Mitra, Rajat, Dasgupta
Format Journal Article
LanguageEnglish
Published Akshantala Enterprises Private Limited 02.02.2015
Subjects
Dengue fever
Diagnosis
Diagnostic equipment (Medical)
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Summary:The performance of a commercial rapid immunochromatographic dengue NS1, IgG/IgM assay device was evaluated against an in-place dengue IgM-capture ELISA in the KPC Medical College & Hospital, Jadavpur. Of the 932 serum samples from patients with clinical diagnosis of acute dengue illness, 542, 237 and 90 samples were tested positive respectively for NS1 ELISA, anti-dengue IgM (MAC ELISA) and anti-dengue IgG ELISA. Comparatively, 476, 205 and 79 samples were tested positive respectively by the immunochromatographic method, has less sensitivity of 13% and a relative specificity of 12% compared to the ELISA method. Though the rapid immunochromato graphic assay device has the advantages of rapid testing which simultaneously detects both IgG and IgM and can also be performed with whole blood, serum or plasma, the user has to exercise extreme caution with the interpretation of the test result. KEYWORDS: Rapid immunochromatographic dengue NS1, NS1 ELISA, MAC ELISA, Flavivirus, DHF, DSS, PCR.
ISSN:2278-4748
2278-4802
DOI:10.14260/jemds/2015/234