Threshold Sensing through a Synthetic Enzymatic Reaction-Diffusion Network
A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reactio...
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| Published in | Angewandte Chemie Vol. 126; no. 31; pp. 8204 - 8207 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English German |
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Weinheim
WILEY-VCH Verlag
28.07.2014
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| Abstract | A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks.
Gemischte Signale: Durch eine Nassstempelmethode können die Konzentrationen von Enzymen und Inhibitoren in schichtartig aufgebauten Gelen räumlich und zeitlich präzise eingestellt werden. Die Kombination der Diffusion von definierten Plätzen mit enzymatischen Reaktionen wie Autokatalyse und Inhibition in einem Hydrogel ergibt ein komplexes biochemisches Netzwerk mit hoher Nachweisempfindlichkeit. |
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| AbstractList | A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks. [PUBLICATION ABSTRACT] Abstract A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks. A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks.Original Abstract: Gemischte Signale: Durch eine Nassstempelmethode konnen die Konzentrationen von Enzymen und Inhibitoren in schichtartig aufgebauten Gelen raeumlich und zeitlich praezise eingestellt werden. Die Kombination der Diffusion von definierten Plaetzen mit enzymatischen Reaktionen wie Autokatalyse und Inhibition in einem Hydrogel ergibt ein komplexes biochemisches Netzwerk mit hoher Nachweisempfindlichkeit. A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks. Gemischte Signale: Durch eine Nassstempelmethode können die Konzentrationen von Enzymen und Inhibitoren in schichtartig aufgebauten Gelen räumlich und zeitlich präzise eingestellt werden. Die Kombination der Diffusion von definierten Plätzen mit enzymatischen Reaktionen wie Autokatalyse und Inhibition in einem Hydrogel ergibt ein komplexes biochemisches Netzwerk mit hoher Nachweisempfindlichkeit. |
| Author | Semenov, Sergey N. de Greef, Tom F. A. Huck, Wilhelm T. S. Markvoort, Albert J. |
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| Copyright | 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
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| Notes | We acknowledge financial support from the European Research Council (ERC; Advanced Grant 246812 Intercom (W.T.S.H.)), the Netherlands Organisation for Scientific Research (NWO; VENI Grant: 722.012.001 (T.F.A.d.G.), VICI Grant 700.10.44 (W.T.S.H.) a Marie Curie Intra-European Fellowship (Grant 300519 (S.N.S.)) and funding from the Ministry of Education, Culture and Science (Gravity program 024.001.035). We acknowledge Dr. V. Chokkalingam for his help with photolithography. Ministry of Education, Culture and Science - No. 024.001.035 European Research Council Netherlands Organisation for Scientific Research - No. 722.012.001; No. 700.10.44 ArticleID:ANGE201402327 ark:/67375/WNG-26M1CSSW-V ERC - No. 246812 istex:7A4E9EE33101B3E3F951A3720C86F3D873D12526 We acknowledge financial support from the European Research Council (ERC; Advanced Grant 246812 Intercom (W.T.S.H.)), the Netherlands Organisation for Scientific Research (NWO; VENI Grant: 722.012.001 (T.F.A.d.G.), VICI Grant 700.10.44 (W.T.S.H.) a Marie Curie Intra‐European Fellowship (Grant 300519 (S.N.S.)) and funding from the Ministry of Education, Culture and Science (Gravity program 024.001.035). We acknowledge Dr. V. Chokkalingam for his help with photolithography. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
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| SubjectTerms | Autocatalysis Biochemische Netzwerke Biochemistry Biosensoren Chemical reactions Chemistry Diffusion Enzymes Enzymkatalyse Inhibitors Nachweisverfahren Networks Reaktions-Diffusions-Systeme Recognition Spatial distribution Stamping |
| Title | Threshold Sensing through a Synthetic Enzymatic Reaction-Diffusion Network |
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