Threshold Sensing through a Synthetic Enzymatic Reaction-Diffusion Network

A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reactio...

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Published inAngewandte Chemie Vol. 126; no. 31; pp. 8204 - 8207
Main Authors Semenov, Sergey N., Markvoort, Albert J., de Greef, Tom F. A., Huck, Wilhelm T. S.
Format Journal Article
LanguageEnglish
German
Published Weinheim WILEY-VCH Verlag 28.07.2014
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
Subjects
Autocatalysis
Biochemische Netzwerke
Biochemistry
Biosensoren
Chemical reactions
Chemistry
Diffusion
Enzymes
Enzymkatalyse
Inhibitors
Nachweisverfahren
Networks
Reaktions-Diffusions-Systeme
Recognition
Spatial distribution
Stamping
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Summary:A wet stamping method to precisely control concentrations of enzymes and inhibitors in place and time inside layered gels is reported. By combining enzymatic reactions such as autocatalysis and inhibition with spatial delivery of components through soft lithographic techniques, a biochemical reaction network capable of recognizing the spatial distribution of an enzyme was constructed. The experimental method can be used to assess fundamental principles of spatiotemporal order formation in chemical reaction networks. Gemischte Signale: Durch eine Nassstempelmethode können die Konzentrationen von Enzymen und Inhibitoren in schichtartig aufgebauten Gelen räumlich und zeitlich präzise eingestellt werden. Die Kombination der Diffusion von definierten Plätzen mit enzymatischen Reaktionen wie Autokatalyse und Inhibition in einem Hydrogel ergibt ein komplexes biochemisches Netzwerk mit hoher Nachweisempfindlichkeit.
Bibliography:We acknowledge financial support from the European Research Council (ERC; Advanced Grant 246812 Intercom (W.T.S.H.)), the Netherlands Organisation for Scientific Research (NWO; VENI Grant: 722.012.001 (T.F.A.d.G.), VICI Grant 700.10.44 (W.T.S.H.) a Marie Curie Intra-European Fellowship (Grant 300519 (S.N.S.)) and funding from the Ministry of Education, Culture and Science (Gravity program 024.001.035). We acknowledge Dr. V. Chokkalingam for his help with photolithography.
Ministry of Education, Culture and Science - No. 024.001.035
European Research Council
Netherlands Organisation for Scientific Research - No. 722.012.001; No. 700.10.44
ArticleID:ANGE201402327
ark:/67375/WNG-26M1CSSW-V
ERC - No. 246812
istex:7A4E9EE33101B3E3F951A3720C86F3D873D12526
We acknowledge financial support from the European Research Council (ERC; Advanced Grant 246812 Intercom (W.T.S.H.)), the Netherlands Organisation for Scientific Research (NWO; VENI Grant: 722.012.001 (T.F.A.d.G.), VICI Grant 700.10.44 (W.T.S.H.) a Marie Curie Intra‐European Fellowship (Grant 300519 (S.N.S.)) and funding from the Ministry of Education, Culture and Science (Gravity program 024.001.035). We acknowledge Dr. V. Chokkalingam for his help with photolithography.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201402327