Characterisation of minor tetra- to hepta-saccharides O-linked to human meconium glycoproteins by t.l.c.-m.s. microsequencing of neoglycolipid derivatives in conjunction with conventional m.s. and 1H-n.m.r. spectroscopy

As part of the establishment of a data base for core and backbone sequences of O-linked oligosaccharides of human meconium glycoproteins, the minor tetra- to hepta-saccharides released from mild-acid treated blood group [corrected] H-active glycoproteins have been studied. These oligosaccharides are...

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Bibliographic Details
Published inCarbohydrate research Vol. 221; p. 191
Main Authors Lawson, A M, Hounsell, E F, Stoll, M S, Feeney, J, Chai, W G, Rosankiewicz, J R, Feizi, T
Format Journal Article
LanguageEnglish
Published Netherlands 16.12.1991
Subjects
Anthocyanins
Carbohydrate Sequence
Chromatography, Thin Layer
Glycolipids - chemistry
Glycoproteins - chemistry
Humans
Infant, Newborn
Magnetic Resonance Spectroscopy
Mass Spectrometry
Meconium - chemistry
Molecular Sequence Data
Oligosaccharides - chemistry
Staining and Labeling
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Summary:As part of the establishment of a data base for core and backbone sequences of O-linked oligosaccharides of human meconium glycoproteins, the minor tetra- to hepta-saccharides released from mild-acid treated blood group [corrected] H-active glycoproteins have been studied. These oligosaccharides are heterogeneous and difficult to isolate, and a t.l.c.-m.s. microsequencing procedure has been applied to the neoglycolipid derivatives, in conjunction with 1H-n.m.r. spectroscopy, methylation analysis, and mass spectrometry (m.s.) of native and methylated oligosaccharides. Among an array of oligosaccharides characterised are those having the branched beta-GlcNAc-(1---6)[beta-Gal- (1---3)]-GalNAcol core, and others with the following linear sequences not characterised previously from this source: beta-Gal-(1---3)-beta-GlcNAc-(1---3)-beta-Gal-(1---3)-GalNAcol, beta-Gal-(1---4)-beta-GlcNAc-(1---3)-beta-Gal-(1---3)-GalNAcol, alpha-GalNAc- (1---3)-beta-Gal-(1---3/4)-beta-GlcNAc-(1---3)-GalNAcol, beta-GlcNAc- (1---3)-beta-Gal-(1---3/4)-beta-GlcNAc-(1---3)- GalNAcol, beta-Gal-(1---3/4)-beta-GlcNAc-(1---3)-beta-Gal-(1---3/4)-beta- GlcNAc-(1---3)-beta-Gal-(1---3)-GalNAcol, and beta-GlcNAc-(1---3)-beta-Gal-(1---3/4)-beta-GlcNAc-(1---3)-beta-Gal- (1---3/4)-beta-GlcNAc-(1---3)-GalNAcol.
ISSN:0008-6215
DOI:10.1016/0008-6215(91)80056-S