Noninvasive in vivo 13C-NMR spectroscopy of a 13C-labeled xenobiotic in the rat

• Published in: Magnetic resonance imaging Vol. 10; no. 6; pp. 975 - 981
• Main Authors: Lanens, D., Muller, H.J., Van de Vyver, F., de Cock-Bunning, Tj, Spanoghe, M., Van der Linden, A., Mulder, G.J., Dommisse, R., Lugtenburg, J.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 1992; Elsevier Science
• Subjects: 13C labeling; 13C NMR; Animals; Biological and medical sciences; Carbon Radioisotopes; Digestive system; Female; In vivo NMR spectroscopy; Injections, Intraperitoneal; Investigative techniques, diagnostic techniques (general aspects); Liver - chemistry; Magnetic Resonance Spectroscopy; Medical sciences; Mitotane - administration & dosage; Mitotane - analogs & derivatives; Mitotane - analysis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Rats; Rats, Wistar; Xenobiotic; Xenobiotics - administration & dosage; Xenobiotics - analysis; 13C labeling; In vivo NMR spectroscopy; 13C NMR; Xenobiotic; Rat; Radiolabelling; Liver; Rodentia; Exploration; NMR spectrometry; Carbon; In vivo; Vertebrata; Mammalia; Animal; Non invasive method; Surface coil
• ISSN: 0730-725X; 1873-5894
• DOI: 10.1016/0730-725X(92)90452-6

This study demonstrates that the xenobiotic product, 1-( o-chlorophenyl)-1-( p-chlorophenyl)-2,2-dichloro-3- 13C-propane can be monitored in the iver of an intact animal by in vivo 13C surface coil NMR spectroscopy after intraperitoneal administration. The carbon-13 label could be detected after a single dose of only 200 mg/kg of the product. The intrahepatic changes of the signal intensity of the labeled product were monitored as a function of time. No signals corresponding to metabolites could be detected.