Detection of 13C labelled compounds by gas chromatography coupled to atomic emission detection--application to caffeine metabolites

This paper illustrates the use of gas chromatography coupled to atomic emission detection (GC-AED) and of the stable isotope tracer 13C for the determination of drug metabolites. After administration of a parent drug labelled with 13C and extraction of the metabolites from the biological fluids, a 1...

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Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 12; no. 2; p. 185
Main Authors Boukraa, M S, Deruaz, D, Bannier, A, Desage, M, Brazier, J L
Format Journal Article
LanguageEnglish
Published England 01.02.1994
Subjects
Caffeine - analogs & derivatives
Caffeine - analysis
Carbon Isotopes
Chromatography, Gas - methods
Gas Chromatography-Mass Spectrometry
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Summary:This paper illustrates the use of gas chromatography coupled to atomic emission detection (GC-AED) and of the stable isotope tracer 13C for the determination of drug metabolites. After administration of a parent drug labelled with 13C and extraction of the metabolites from the biological fluids, a 13C chromatographic profile is determined using the specific detection of the 13C atomic emission and subtraction of the 13C natural abundance. Thus, only the compounds which are metabolites with a 13C enrichment over the natural abundance are detected. [1,3,7 trimethyl-13C3]xanthine which is extensively metabolized by the liver is used as an example.
ISSN:0731-7085
DOI:10.1016/0731-7085(94)90029-9