Procedure for red blood cell labelling with 99mTc-HMPAO. Methodology and quality control

We present here results on the labelling of red blood cells with 99mTc-HMPAO as an alternative method to the usual in vitro technique. Anticoagulant agents, the labelling medium with plasma, and the lapse of time between 99mTc-HMPAO preparation and labelling are the main factors which affect the eff...

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Published inNuclear medicine and biology Vol. 21; no. 7; pp. 963 - 967
Main Authors Bassa, P., Garzón, J.R.García, Piera, C., Pavía, A., Minoves, M., Moragas, M., Pavía, J., Lomeña, F., Setoain, J.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier Inc 01.10.1994
Elsevier
Subjects
Biological and medical sciences
Contrast media. Radiopharmaceuticals
Erythrocytes
Heart - diagnostic imaging
Humans
Medical sciences
Organotechnetium Compounds
Oximes
Pharmacology. Drug treatments
Quality Control
Radionuclide Imaging
Technetium Tc 99m Exametazime
Radionuclide study
Radiolabelling
Quality control
Red blood cell
Technetium
Scintigraphy
Technique
In vitro
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Summary:We present here results on the labelling of red blood cells with 99mTc-HMPAO as an alternative method to the usual in vitro technique. Anticoagulant agents, the labelling medium with plasma, and the lapse of time between 99mTc-HMPAO preparation and labelling are the main factors which affect the efficiency of the procedure. A 93.9 ± 2.3% labelling yield was obtained with freshly prepared 99mTc-HMPAO. In vitro (tracer elution of 4.3 ± 1.2% at 60 mins) and in vivo (percentage of plasma activity at 60 mins, 7.8 ± 2.8%) stability of the label, as well as image quality, qualify 99mTc-HMPAO labelled red blood cells as a suitable agent for clinical use.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0969-8051
1872-9614
DOI:10.1016/0969-8051(94)90086-8