Generation of Induced Cardiospheres via Reprogramming of Mouse Skin Fibroblasts

Cardiospheres represent a more effective cell-based therapy for treatment of myocardial infarction than stem cells of non-cardiac origin. Unfortunately, their therapeutic application is limited by low yield of cell harvesting, declining quality and quantity during the aging process, and the need for...

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Bibliographic Details
Published inCurrent protocols in stem cell biology Vol. 46; no. 1; p. e59
Main Authors Lian, Wei, Jia, Yuning, Li, Lingyun, Huang, Zhong, Xu, Jianyong
Format Journal Article
LanguageEnglish
Published United States 01.08.2018
Subjects
cardiac progenitor cells
induced cardiosphere
cardiospheres
somatic reprogramming
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Summary:Cardiospheres represent a more effective cell-based therapy for treatment of myocardial infarction than stem cells of non-cardiac origin. Unfortunately, their therapeutic application is limited by low yield of cell harvesting, declining quality and quantity during the aging process, and the need for highly invasive heart biopsy. Therefore, there is an emerging interest in generating cardiosphere-like stem cells from somatic cells via somatic reprogramming. This novel approach would provide an unlimited source of stem cells with cardiac differentiation potential. Here we provide the detailed protocol for generating induced cardiospheres (iCS) for cardiac regeneration by somatic reprogramming of mouse fibroblasts using a panel of pluripotent transcription factors and cardiotrophic growth factors. © 2018 by John Wiley & Sons, Inc.
ISSN:1938-8969
DOI:10.1002/cpsc.59