Using Evans Blue Dye to Determine Blood-Brain Barrier Integrity in Rodents

The blood-brain barrier (BBB) is an active and selective barrier that shields the brain from endogenous and exogenous insults. Different stimuli may lead to the disruption of this barrier, including inflammation and trauma. Several methods are used to evaluate BBB disruption. The most widely used me...

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Bibliographic Details
Published inCurrent protocols in immunology Vol. 126; no. 1; p. e83
Main Authors Goldim, Mariana Pereira de Souza, Della Giustina, Amanda, Petronilho, Fabricia
Format Journal Article
LanguageEnglish
Published United States 01.09.2019
Subjects
blood-brain barrier
brain
Evans blue
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Summary:The blood-brain barrier (BBB) is an active and selective barrier that shields the brain from endogenous and exogenous insults. Different stimuli may lead to the disruption of this barrier, including inflammation and trauma. Several methods are used to evaluate BBB disruption. The most widely used method is Evans blue (EB) dye extravasation. EB cannot normally pass through the BBB and thus its presence in brain tissue indicates alterations in permeability. This protocol details the steps of EB extravasation in rodents. Important aspects regarding critical steps and advantages are also provided. © 2019 by John Wiley & Sons, Inc.
ISSN:1934-368X
DOI:10.1002/cpim.83