UBA domain protein SUF1 interacts with NatA‐complex subunit NAA15 to regulate thermotolerance in Arabidopsis

• Published in: Journal of integrative plant biology Vol. 64; no. 7; pp. 1297 - 1302
• Main Authors: Song, Ze‐Ting, Chen, Xiao‐Jie, Luo, Ling, Yu, Feifei, Liu, Jian‐Xiang, Han, Jia‐Jia
• Format: Journal Article
• Language: English
• Published: China (Republic : 1949- ) Wiley Subscription Services, Inc 01.07.2022; State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310027,China; Laboratory of Ecology and Evolutionary Biology,State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650500,China%Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology,Institute of Biodiversity,School of Ecology and Environmental Science,Yunnan University,Kunming 650500,China%Laboratory of Ecology and Evolutionary Biology,State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650500,China%College of Grassland Science and Technology,China Agricultural University,Beijing 100083,China%State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310027,China%State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310027,China; Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology,Institute of Biodiversity,School of Ecology and Environmental Science,Yunnan University,Kunming 650500,China; Laboratory of Ecology and Evolutionary Biology,State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650500,China
• Subjects: Acetyltransferase; Arabidopsis; basal thermotolerance; Heat; Heat stress; Heat tolerance; Mutants; NAA15; NatA complex; Proteasomes; Protein folding; Proteins; Substrates; SUF1; Temperature tolerance; UBA‐domain protein; Ubiquitin; Ubiquitin-protein ligase; heat stress; SUF1; NAA15; NatA complex; basal thermotolerance; UBA-domain protein
• ISSN: 1672-9072; 1744-7909
• DOI: 10.1111/jipb.13273

Summary During recovery from heat stress, plants clear away the heat‐stress‐induced misfolded proteins through the ubiquitin‐proteasome system (UPS). In the UPS, the recognition of substrate proteins by E3 ligase can be regulated by the N‐terminal acetyltransferase A (NatA) complex. Here, we determined that Arabidopsis STRESS‐RELATED UBIQUITIN‐ASSOCIATED‐DOMAIN PROTEIN FACTOR 1 (SUF1) interacts with the NatA complex core subunit NAA15 and positively regulates NAA15. The suf1 and naa15 mutants are sensitive to heat stress; the NatA substrate NSNC1 is stabilized in suf1 mutant plants during heat stress recovery. Therefore, SUF1 and its interactor NAA15 play important roles in basal thermotolerance in Arabidopsis. During recovery from heat stress, plants clear away heat‐stress‐induced misfolded proteins through the ubiquitin‐proteasome system (UPS) and the N‐terminal acetyltransferase A (NatA) complex regulates the recognition of UPS substrate proteins. STRESS‐RELATED UBIQUITIN‐ASSOCIATED‐DOMAIN PROTEIN FACTOR1 interacts with the NatA complex and plays important roles in basal thermotolerance in Arabidopsis.