TARGETING THE TUMOR METABOLISM BY OXAMATE POTENTIATES THE IMPACT OF CHEMOTHERAPEUTICS IN COLORECTAL CANCER CELLS

Purpose: Cancer cells promote lactate formation via pyruvate rather than oxidative phosphorylation by programming their metabolism to maintain proliferation under the Warburg effect. It has shown that the altered metabolic phenotype with activation of lactate dehydrogenase-A in the cancer cell may a...

Full description

Saved in:
Bibliographic Details
Published inJournal of Basic and Clinical Health Sciences Vol. 5; no. 3; p. 205
Main Authors Calibasi-Kocal, Gizem, Cakici, Cagri, Toksoz, Feriha, Inanc-Surer, Seniz, Sever, Tolga, Basbinar, Yasemin, Yigitbasi, Turkan
Format Journal Article
LanguageEnglish
Published AVES 01.09.2021
Subjects
Analysis
Cancer
Cancer cells
Care and treatment
Chemotherapy
Colorectal cancer
Fluorouracil
Metastasis
Oncology, Experimental
Online AccessGet full text

Cover

More Information
Summary:Purpose: Cancer cells promote lactate formation via pyruvate rather than oxidative phosphorylation by programming their metabolism to maintain proliferation under the Warburg effect. It has shown that the altered metabolic phenotype with activation of lactate dehydrogenase-A in the cancer cell may affect survival, chemotherapy resistance, and metastasis. In this direction, studies are focusing on reprogramming cancer metabolism and increase the effectiveness of chemotherapy. In this study, the main aim was to target the Warburg phenotype via the inhibition of lactate dehydrogenase with the combination of sodium oxamate and current colorectal cancer treatment options such as 5-fluorouracil and irinotecan. Methods: The effect of chemotherapeutics on the cellular behavior was evaluated by real-time cytotoxicity and migration analysis systems, and metabolic phenotype was assessed by measuring lactate, lactate dehydrogenase expression, and reactive oxygen species levels. Results: According to the results, the viability and migration of colorectal cancer cells were significantly decreased with the combination of chemotherapeutics and sodium oxamate which decreases lactate levels. Conclusion: As a result, the combination of sodium oxamate with chemotherapeutics hinders the cancer cell viability and migration by changing metabolic phenotype with decreased lactate. Keywords: Colorectal cancer, tumor metabolism, lactate, sodium oxamate, Warburg effect
ISSN:2458-8938
DOI:10.30621/jbachs.988996