The structural characterization and biological activity of sulfamethoxazolyl-azo-p-cresol, its copper(II) complex and their theoretical studies

• Published in: New journal of chemistry Vol. 40; no. 6; pp. 5019 - 5031
• Main Authors: Sahu, Nilima, Das, Dipankar, Mondal, Sudipa, Roy, Suman, Dutta, Paramita, Sepay, Nayim, Gupta, Suvroma, Lopez-Torres, Elena, Sinha, Chittaranjan
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 2016
• Subjects: Chemistry; Chemistry, Multidisciplinary; Physical Sciences; Science & Technology; MOLECULAR CALCULATIONS; EFFECTIVE CORE POTENTIALS; CRYSTAL-STRUCTURE; DRUG DISCOVERY; DNA INTERACTION; ENERGIES; SULFONAMIDE RESISTANCE; ANTIMICROBIAL ACTIVITY; FLUORESCENT-PROBE; TRIMETHOPRIM-SULFAMETHOXAZOLE
• ISSN: 1144-0546; 1369-9261
• DOI: 10.1039/c5nj02983j

(E)-4-((2-Hydroxy-5-methylphenyl)diazenyl)-N-(5-methylisoxazole-3-yl)benzene sulfonamide (SMX-N=N-C6H3(p-Me)-OH, 1) and its Cu(II) complex, [Cu(SMX-NQN-C6H3(p-Me)-O)(2)](n) (2), have been characterized by spectroscopic data and single crystal X-ray diffraction studies. The supramolecular 1D chain of 1 is constituted by inter-and intra-molecular hydrogen bonds and also by pi center dot center dot center dot pi interactions of aromatic rings. Complex 2 has a tetragonally distorted octahedral structure in which ligand 1 serves as an N, O chelator and forms a planar Cu(N, O)(2) motif; two axial positions are occupied by the oxazolyl-N of neighbouring units and a 3D structure is formed. The biological activities of these compounds have been evaluated against Gram positive (B. subtilis; IC50: 105 mu g ml(-1) (1) and 105 mu g ml(-1) (2)) and Gram negative bacteria (E. coli; IC50: 66.36 mu g ml(-1) (1) and 62.2 mu g ml(-1) (2)) and the complexes exhibit better efficiency. Interactions of DNA with 1 and 2 have been examined and the binding constants are K-b(1), 5.920 x 10(4) M-1 and K-b(2), 4.445 x 10(4) M-1. The in silico test is used to predict the most favoured binding mode of 1 and 2 with the active site residues of DHPS (dihydropteroate synthetase) of E. coli and of DNA. The Cu(II) complex (2) binds more efficiently (CDOCKER energy, 2, -61.35 a.u.) in the DHPS cavity than ligand 1 (CDOCKER energy, 1, -43.90 a.u.). The electronic structures and spectral properties of 1 and 2 have been investigated by DFT and TD-DFT computation of optimized geometries of the compounds.