Gold nanoparticle–mesoporous silica sheet composites with enhanced antibody adsorption capacity
A notably high-capacity antibody adsorbent was prepared using dispersed gold nanoparticles immobilised on a mesoporous silica sheet. The mesoporous silica sheet (MPS sheet) was prepared via a dual-templating method using tetraethoxysilane and 3-aminopropyltriethoxysilane as the silica framework mate...
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Published in | New journal of chemistry Vol. 39; no. 5; pp. 4070 - 4077 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
01.01.2015
|
Online Access | Get full text |
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Summary: | A notably high-capacity antibody adsorbent was prepared using dispersed gold nanoparticles immobilised on a mesoporous silica sheet. The mesoporous silica sheet (MPS sheet) was prepared
via
a dual-templating method using tetraethoxysilane and 3-aminopropyltriethoxysilane as the silica framework materials. Gold nanoparticles were loaded onto the amino-functionalised surface of the MPS sheet
via
deposition–precipitation using HAuCl
4
as the Au colloid precursor and then crystallised
via
calcination. The properties of the gold nanoparticle–silica composite and the dispersion of the nanoparticles were characterised using transmission electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction analysis. The surface characteristics of gold nanoparticle composite materials calcined at different temperatures were also evaluated using Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and thermogravimetry data. The silica–gold nanoparticle composite sheet crystallised at 550 °C (sheet-Au-550) was mesoporous in character (pore diameter = 3.8 nm) with a BET surface area of 258.0 m
2
g
−1
. With respect to antibody adsorption, the sheet materials had a relatively higher capacity than the typical cylindrical mesoporous silica, and sheet-Au-550 exhibited a strong performance (maximum adsorption quantity
Q
Max
= 0.26 mg IgG per mg carrier). In addition, the IgG adsorption equilibrium for sheet-Au-550 fits the Langmuir isotherm model better than the Freundlich plot model. These results will be very useful for the design of adsorption materials for biomolecules, such as antibodies. |
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ISSN: | 1144-0546 1369-9261 |
DOI: | 10.1039/C5NJ00033E |