Regeneration of industrial sugarcane using in-vitro plant apical meristem

A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of r...

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Published inElectronic journal of plant breeding Vol. 9; no. 4; pp. 1342 - 1347
Main Authors Ajadi, A., Ehirim, B.O., Ishaq, M. N., Isah, A., Isong, A., Ghali, A., Kester, O., Ejizu, A. N.
Format Journal Article
LanguageEnglish
Published Indian Society of Plant Breeders 2018
Subjects
bap
culture
iba
meristem shoots
naa
proliferation
sugarcane
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Summary:A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of regenerated shoots were obtained from MS medium supplemented with BAP (0.3mg/l) and NAA (0.03mg/l)giving an average of 8 shoot per meristem shoot cultured. Roots were induced using MS medium supplemented with different combinations of NAA and IBA. NAA (3.0mg/l) gave highest rooting percentage of 94.74. The meristem shoot tip demonstrated good response to plant regeneration of sugarcane variety NCS 008 through in-vitro culture.
ISSN:0975-928X
0975-928X
DOI:10.5958/0975-928X.2018.00167.9