Regeneration of industrial sugarcane using in-vitro plant apical meristem
A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of r...
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Published in | Electronic journal of plant breeding Vol. 9; no. 4; pp. 1342 - 1347 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Indian Society of Plant Breeders
2018
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Subjects | |
Online Access | Get full text |
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Summary: | A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of regenerated shoots were obtained from MS medium supplemented with BAP (0.3mg/l) and NAA (0.03mg/l)giving an average of 8 shoot per meristem shoot cultured. Roots were induced using MS medium supplemented with different combinations of NAA and IBA. NAA (3.0mg/l) gave highest rooting percentage of 94.74. The meristem shoot tip demonstrated good response to plant regeneration of sugarcane variety NCS 008 through in-vitro culture. |
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ISSN: | 0975-928X 0975-928X |
DOI: | 10.5958/0975-928X.2018.00167.9 |