Evaluation of antioxidant enzymes changes in synovial fluid and blood, following experimental osteoarthritis in dogs

• Published in: Comparative clinical pathology Vol. 24; no. 1; pp. 23 - 27
• Main Authors: Nikahval, Behrooz, Hosseini, Zahra, Nazifi, Saeed, Foroud, Mehrzad
• Format: Journal Article
• Language: English
• Published: London Springer London 2015; Springer Nature B.V
• Subjects: Hematology; Medicine; Medicine & Public Health; Oncology; Original Article; Pathology; Antioxidant; Synovial fluid; Osteoarthritis
• ISSN: 1618-5641; 1618-565X
• DOI: 10.1007/s00580-013-1848-8

Osteoarthritis (OA) is a degenerative process which involves different structures of the synovial joints. Reactive oxygen species may participate in the initiation and progression of osteoarthritis. This study examined potential changes in the activities of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX)) in synovial fluid and blood, and estimated their relationship to the degree of lipid peroxidation evaluated by malondialdehyde (MDA) concentration, following osteoarthritis in dogs. Osteoarthritis was induced in five mature dogs by transaction of cranial cruciate ligament. Blood and synovial samples were gained once before surgery—as baseline—and on days 14, 28, 90, and 180, postoperatively. Antioxidants and MDA levels were evaluated on these days. In SOD, GPX, CAT, and MDA concentrations in blood there were no significant ( P  < 0.05) changes between the different times. SOD, GPX, CAT, and MDA levels in synovial fluid were significantly ( P  < 0.05) higher 2 and 4 weeks after induction of OA, compared to other times. In conclusion, this study displayed higher antioxidants and MDA levels in early stages of OA as inflammatory phase compared to other times in synovial fluid. However, measurement of these enzymes and MDA in blood did not show any changes.