Effects of Hypoxic Preconditioning on Oxidant-Antioxidant Systems in Rat Lung

Objective: Hypoxic preconditioning allows cells to gain resistance to hypoxic damage. There are a limited number of studies suggesting that hypoxic preconditioning increases antioxidant capacity in the lung. In this study, we aimed to evaluate effects of hypoxic preconditioning on oxidant/antioxidan...

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Published inTürkiye klinikleri. Türkiye klinikeri journal of medical sciences. Tıp bilimleri dergisi Vol. 39; no. 2; pp. 194 - 201
Main Authors KILINÇ, Sevtap, GÜNEY, Sevin
Format Journal Article
LanguageEnglish
Published Balgat Ortadogu Reklam Tanitim Yayincilik Turizm Egitim Insaat Sanayi ve Ticaret A.S 2019
Subjects
Antioxidants
Hypoxia
Ischemia
Kinases
Lipid peroxidation
Lipids
Lungs
Musculoskeletal system
Oxidative stress
Physiology
University faculty
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Summary:Objective: Hypoxic preconditioning allows cells to gain resistance to hypoxic damage. There are a limited number of studies suggesting that hypoxic preconditioning increases antioxidant capacity in the lung. In this study, we aimed to evaluate effects of hypoxic preconditioning on oxidant/antioxidant systems in rat lung. Material and Methods: Rats were divided into 4 groups: control, preconditioning (PC) (10% O2), severe hypoxia (SH) (7% O2) and PC + SH. The parameters related to oxidative stress and antioxidant defense mechanisms, which are malondialdehyde (MDA) levels, total oxidant system (TOS), total antioxidant capacity (TAC), oxidative stress index (OSI), superoxide dismutase (SOD) and glutathione (GSH) activity, were measured. Results were evaluated using the One-Way ANOVA and t-test; p0.05 was considered significant. Results: Compared with the control group, MDA levels decreased in all hypoxic groups; decrements in PC and SH groups were statistically significant. Compared with the control group, levels of TOS showed a significant increase in the PC+SH group. OSI of PC+SH group was significantly higher than other groups. There was no significant difference in TAC levels between the groups. Compared to other groups, SOD activity showed a significant decrease in the PC+SH group. GSH levels showed a significant decrease in PC+SH group compared to both control and PC group. Conclusion: Our findings suggest that hypoxic preconditioning does not have an effect on antioxidant defense systems in lungs, but severe hypoxia does affect oxidant/antioxidant systems.
ISSN:1300-0292
2146-9040
DOI:10.5336/medsci.2018-62968