Biological resorption of fibers from chitosan in endomysium and perimysium of muscular tissue

• Published in: Cell and tissue biology Vol. 10; no. 5; pp. 395 - 401
• Main Authors: Dobrovol’skaya, I. P., Popryadukhin, P. V., Yudin, V. E., Ivan’kova, E. M., Yukina, G. Yu, Yudenko, A. N., Smirnova, N. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.09.2016; Springer Nature B.V
• Subjects: Biocompatibility; Biomedical and Life Sciences; Cell Biology; Cell proliferation; Chitosan; Electron microscopy; Life Sciences; Localization; Mesenchyme; Resorption; Scanning electron microscopy; Stem cell transplantation; Stem cells; mesenchyme stem cells; resorption; tissue engineering; perimysium of muscular tissue; chitosan fibers; endomysium
• ISSN: 1990-519X; 1990-5203
• DOI: 10.1134/S1990519X16050047

The resorption of fibers from chitosan implanted into emdomysium and perimysium of the rat’s broadest muscle of the back is comparatively studied in vivo by the scanning electron microscopy and histologic analysis methods. It is shown that the mechanism and rate of resorption of the fibers from chitosan depend on the fiber localization in the muscular tissue. Implantation of chitosan fibers into endomysium, where they have been in direct contact with muscle fibers, results in 14 days in the formation of transverse cracks, fiber fragmentation, and their partial resorption. Complete resorption of fibers in endomysium is observed in 30 days. Fibers implanted into perimysium maintain integrity in 7 days of the experiment, and a fibrous tissue is formed around the fibers. There is no destruction of chitosan fibers in 45 days of the exposition. The biocompatibility of the chitosan fibers is confirmed by the effective adhesion and proliferation mesenchyme stem cells on their surface.