Possibility for Application of Extracellular Protease of Micromycete Aspergillus ochraceus for Determining Factor X Content in Human Blood Plasma

It has been shown that factor X activity determined in normal plasma using Aspergillus ochraceus protease is comparable with the activity determined with a commercial analogue, a protease from Russell’s viper venom (RVV-X ® ). It was revealed that the protease of A. ochraceus , along with the RVV-X...

Full description

Saved in:
Bibliographic Details
Published inMoscow University biological sciences bulletin Vol. 74; no. 2; pp. 117 - 120
Main Authors Orekhova, A. V., Osmolovskiy, A. A., Kreyer, V. G., Baranova, N. A., Egorov, N. S.
Format Journal Article
LanguageEnglish
Published Moscow Pleiades Publishing 01.04.2019
Springer Nature B.V
Subjects
Aspergillus ochraceus
Biochemistry
Biomedical and Life Sciences
Cell Biology
Coagulation factors
Life Sciences
Plant Sciences
Short Report
Snakes
Venom
Zoology
proteolytic enzymes
pathological plasma
activators of factor X
aspergilli protease
RVV-X
diagnostics of factor X
Online AccessGet full text

Cover

More Information
Summary:It has been shown that factor X activity determined in normal plasma using Aspergillus ochraceus protease is comparable with the activity determined with a commercial analogue, a protease from Russell’s viper venom (RVV-X ® ). It was revealed that the protease of A. ochraceus , along with the RVV-X ® preparation, can be used to determine the content of factor X in the plasma with its reduced content. A study of the protease activity of A. ochraceus as an activator to factor X showed that it is slightly higher compared to the snake venom preparation, which can make A. ochraceus protease a promising substitute for the snake activator in diagnostics for determining the content of factor X.
ISSN:0096-3925
1934-791X
DOI:10.3103/S0096392519020081