Bone morphogenetic protein 2: heterologous expression and potential in bone regeneration

Currently, bone morphogenetic protein 2 (BMP-2) is one of the two osteoinductive growth factors used in medical devices to promote bone formation. Typically, this protein is bought from commercial houses at high rates and in small quantities that are not enough to cover clinical needs. Because of th...

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Bibliographic Details
Published inActualidades Biológicas Vol. 43; no. 114; pp. 1 - 10
Main Authors Mesa Restrepo, Andrea, Fernando Alzate, Juan, Patiño Gonzalez, Edwin Bairon
Format Journal Article
LanguageEnglish
Portuguese
Published Medellín Universidad de Antioquía 01.06.2021
Instituto de Biología, Universidad de Antioquia
Universidad de Antioquia
Subjects
alkaline phosphatase
Biological activity
BIOLOGY
Bone growth
Bone morphogenetic protein 2
Bone morphogenetic proteins
Bones
Cell lines
cells c2c12
Collagen
DNA polymerase
Enzymes
Fractures
hbmp‐2
Kinases
Ligands
Medical equipment
Mineralization
Phosphorylation
Proteins
refolding protein
Regeneration
rhBMP-2
Alkaline phosphatase
Células C2C12
Cells C2C12
Refolding protein
Fosfatasa alcalina
Renaturalización
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Summary:Currently, bone morphogenetic protein 2 (BMP-2) is one of the two osteoinductive growth factors used in medical devices to promote bone formation. Typically, this protein is bought from commercial houses at high rates and in small quantities that are not enough to cover clinical needs. Because of this, it has been proposed that research centers use their own heterologous expression systems to have a constant supply of BMP-2. The aim of this study was to standardize the heterologous expression of BMP-2 and evaluate its osteoinductive activity in vitro. Our procedure for expression and purification was based on recombinant DNA technology using the plasmid pET-28 and IPTG as inductor. After extracting the protein from inclusion bodies, folding it and modifying it via a redox system, we observed via electrophoresis a 26 kDa dimer. We evaluated its osteoinductive activity in myoblastic C2C12 by quantifying enzymatically the activity of alkaline phosphate (ALP) and staining mineralization nodules. ALP activity is proportional to BMP-2 concentration, increasing 90% at 3 µg/mL. These cells form calcium nodules, mineralizing 50% of the area.
ISSN:0304-3584
2145-7166
DOI:10.17533/udea.acbi.v43n114a01