The pollen dispersal ability for cross-pollination in winter wheat (Triticum aestivum L.) is related to anther extrusion capability rather than to pollen output

• Published in: South African journal of botany Vol. 148; pp. 283 - 292
• Main Authors: Denisow, Bożena, Masierowska, Marzena, Winiarczyk, Krystyna, Rakoczy-Trojanowska, Monika
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2022
• Subjects: Anther release; Pollen germination; Pollen grains per anther; Pollen grains per spike; Pollen grains per spike; Anther release; Pollen grains per anther; Pollen germination
• ISSN: 0254-6299
• DOI: 10.1016/j.sajb.2022.04.031

Triticum aestivum is a self-pollinated species with a high proportion of cleistogamous flowers constituting a blockade for out-crossing. Therefore, male parent characteristics are the main goal in the production of hybrid wheat seeds. In a two-year study conducted in Lublin (Poland), the features of flowering biology that can be relevant for the cross-pollination ability of the pollen donor were investigated in winter wheat genotypes. In particular, the anther extrusion capacity, pollen production at the anther and spike levels, and pollen biological value were examined. The pollen production in the anthers was established using an electronic particle counter. The pollen biological value was assessed based on viability (acetocarmine staining) and in vitro pollen germination (raffinose medium) assays. The inter-genotype effect for anther extrusion was significantly correlated to the post-anthesis filament length. The anther extrusion for the individual genotypes fluctuated significantly between the years of the study; however, the interaction with meteorological factors was not assessed. The considerable inter-genotype variability in the pollen donor capability for cross-pollination was highly significantly correlated with the proportion of extruded anthers rather than pollen production, indicating that anther extrusion plays a more important role in the contribution to the final amount of pollen available for cross pollination. The pollen viability determined by staining was high (between 97.5% and 100%), while the in vitro pollen germination varied substantially among the genotypes (between 17.3% and 87.8%). The pollen dispersal ability for cross-pollination in wheat can be improved mainly by increasing the proportion of extruded anthers; however, the pollen production and pollen germination potential cannot be ignored if complete characterization of the ideal male idiotype is to be achieved. [Display omitted] •Proportion of extruded anthers plays substantial role for cross-pollination in wheat.•Anther extrusion is mainly related to the post-anthesis filament length.•Pollen production and pollen germination are important for the ideal male idiotype.