Assessment of variable agroclimatic impact on dissipation kinetics of ready-mix fungicide formulation in green chili for harmonization of food safety

• Published in: Journal of food composition and analysis Vol. 110; p. 104541
• Main Authors: Mandal, Swagata, Poi, Rajlakshmi, Hazra, Dipak Kumar, Bhattacharyya, Sudip, Banerjee, Hemanta, Karmakar, Rajib
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.07.2022
• Subjects: Chili; LC-MS/MS; Mancozeb; Tricyclazole; UV-Vis spectrophotometer; Chili; LC-MS/MS; UV-Vis spectrophotometer; Mancozeb; Tricyclazole
• ISSN: 0889-1575; 1096-0481
• DOI: 10.1016/j.jfca.2022.104541

Multi-locational supervised field trials were conducted with ready-mix formulation of two fungicides, tricyclazole (18%) + mancozeb (62%) in green chili (Capsicum annuum) to assess the impacts of variable weather conditions on the residual profile for more realistic food safety evaluation. Liquid chromatography-Electrospray Ionization-Mass Spectrometry [LC-ESI(+ve)-MS/MS] and Ultraviolet Visible (UV-Vis) spectrophotometer have been employed for tricyclazole and mancozeb determination respectively. Tricyclazole was extracted in acetone: water (9:1) with dispersive solid phase extraction (d-SPE) cleaned up whereas mancozeb was digested in concentrated HCl and SnCl2. The developed method was successfully validated as per SANTE/12682/2019 guidelines. On green chilli, 225 + 775 g a.iha−1(active ingredient per hectare) (1250 g formulation-ha−1) and 450 + 1550 g a.iha−1 (active ingredient per hectare) (2500 g formulation-ha−1) of a combined formulation of tricyclazole (18%) and mancozeb (62%), WP, were employed as the recommended dose (T1) and double the recommended dose (T2), respectively. The initial depositions of tricyclazole were varied from 0.47 to 0.74 and 1.30–2.33 mg kg−1 and for mancozeb ranged from 0.93 to 1.31 and 2.68–4.13 mg kg−1 at the respective doses. The respective half-life (days) values of tricyclazole and mancozeb were 1.65–2.41 and 1.20–1.66 irrespective of doses and locations. The maximum residue limit (MRL) of 0.05 mg kg−1 for tricyclazole in green chili has been proposed for Food Safety and Standard Authority of India (FASSAI), India with recommended pre-harvest intervals (PHI) of 12 days. The third location (Anand Agricultural University, Anand, Gujarat) with the least rainfall and humidity has recorded the highest initial deposition, persistence, half-life, and PHI. The ready-mix fungicide has also increased fresh yield up to 22–34%. All these data may provide valuable information on the safe use and promotion of fungicides mixture. [Display omitted] •Dissipation kinetics of fungicides mixture in green chili followed 1st order reaction.•Tricyclazole was analyzed in LC-MS/MS with LOD 0.003 mg kg-1 and Mancozeb in a spectrophotometer with LOD 0.03 mg kg-1.•Third location with the least rainfall, humidity has recorded highest initial deposition, half-life and PHI.•Fresh yield increased upto 22%-34% due to application.•MRL of tricyclazole in green chili 0.05 mg kg−1 (FSSAI, India) has been proposed PHI 12 (days) for mixed fungicides.