Study of cell culture strains sensitivity to sacbrood virus
Abstract A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. T...
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Published in | IOP conference series. Earth and environmental science Vol. 677; no. 4; p. 42031 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
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01.03.2021
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Abstract | Abstract A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. This work was carried out in the Department of Cell Biotechnology and the Laboratory of Bee Diseases of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”. For the study, we took cell cultures of the African green monkey kidneys (Vero (3 strains) - Vero (strain from the FSC VIEV Collection)), Vero K (strain from the FSC VIEV Collection), Vero (strain from Germany). We have shown that all 3 of the Vero cell cultures above strains are sensitive to SBV, however, the cell model in which the most pronounced signs of CPD were revealed is the Vero K cell line. The first signs of CPD manifestation virus in the culture of Vero K cells were observed at first passage. After 6 days. screeds were found in the monolayer after infection. At the second passage of the virus after 5 days. culturing CPD manifested itself as the appearance of voids, cell strands, and rounding of the cells part. |
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AbstractList | Abstract A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. This work was carried out in the Department of Cell Biotechnology and the Laboratory of Bee Diseases of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”. For the study, we took cell cultures of the African green monkey kidneys (Vero (3 strains) - Vero (strain from the FSC VIEV Collection)), Vero K (strain from the FSC VIEV Collection), Vero (strain from Germany). We have shown that all 3 of the Vero cell cultures above strains are sensitive to SBV, however, the cell model in which the most pronounced signs of CPD were revealed is the Vero K cell line. The first signs of CPD manifestation virus in the culture of Vero K cells were observed at first passage. After 6 days. screeds were found in the monolayer after infection. At the second passage of the virus after 5 days. culturing CPD manifested itself as the appearance of voids, cell strands, and rounding of the cells part. A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. This work was carried out in the Department of Cell Biotechnology and the Laboratory of Bee Diseases of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”. For the study, we took cell cultures of the African green monkey kidneys (Vero (3 strains) - Vero (strain from the FSC VIEV Collection)), Vero K (strain from the FSC VIEV Collection), Vero (strain from Germany). We have shown that all 3 of the Vero cell cultures above strains are sensitive to SBV, however, the cell model in which the most pronounced signs of CPD were revealed is the Vero K cell line. The first signs of CPD manifestation virus in the culture of Vero K cells were observed at first passage. After 6 days. screeds were found in the monolayer after infection. At the second passage of the virus after 5 days. culturing CPD manifested itself as the appearance of voids, cell strands, and rounding of the cells part. |
Author | Kalinin, A G Kuleshov, K V Isaev, Y G |
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Cites_doi | 10.1155/2011/212146 10.1371/journal.pone.0077263 10.1016/j.virusres.2014.12.008 10.1111/j.1744-7348.1983.tb02735.x 10.1016/j.jip.2006.03.010 10.18821/0208-0613-2017-35-1-31-35 10.1128/genomeA.00076-12 |
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References | Grobov (EES_677_4_042031bib16) 1987 Kweon (EES_677_4_042031bib25) 2015; 198 Kalashnikov (EES_677_4_042031bib21) 2012; 1 Dyakonov (EES_677_4_042031bib6) 2011 Kalashnikov (EES_677_4_042031bib23) 2017; 1 Grobov (EES_677_4_042031bib20) 2006; 7 Flenniken (EES_677_4_042031bib18) 2013; 8 Dyakonov (EES_677_4_042031bib4) 2005 Ma (EES_677_4_042031bib12) 2011; 1 Kalashnikov (EES_677_4_042031bib22) 2014; 1 Nguyen (EES_677_4_042031bib14) 2013; 1 Taranov (EES_677_4_042031bib10) 2014; 11 Batuev (EES_677_4_042031bib2) 1996; 2 Galnbek (EES_677_4_042031bib3); 10 Bailey (EES_677_4_042031bib11) 1983; 103 Khangalova (EES_677_4_042031bib8) 2008 Chen (EES_677_4_042031bib17) 2006; 92 Sotnikov (EES_677_4_042031bib24) 2020; 421 Ma (EES_677_4_042031bib13) 2013; 8 Sayfutdinova (EES_677_4_042031bib7) 2013; 4 Rana (EES_677_4_042031bib15) 2011; 10 Dyakonov (EES_677_4_042031bib5) 2009 Mader Monaghan (EES_677_4_042031bib9) 2011 Zhang (EES_677_4_042031bib19) 2016 (EES_677_4_042031bib1) 2019 |
References_xml | – start-page: 408 year: 2005 ident: EES_677_4_042031bib4 article-title: Hybrid animal cell cultures in virology and biotechnology contributor: fullname: Dyakonov – volume: 2 start-page: 34 year: 1996 ident: EES_677_4_042031bib2 article-title: Viral diseases of bees publication-title: Beekeeping contributor: fullname: Batuev – year: 2019 ident: EES_677_4_042031bib1 – volume: 1 start-page: 1 year: 2011 ident: EES_677_4_042031bib12 article-title: Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate publication-title: Comparative and Functional Genomics doi: 10.1155/2011/212146 contributor: fullname: Ma – volume: 8 start-page: 1 year: 2013 ident: EES_677_4_042031bib18 article-title: Non-Specific dsRNA-Mediated Antiviral Response in the Honey Bee publication-title: PLoS ONE doi: 10.1371/journal.pone.0077263 contributor: fullname: Flenniken – volume: 198 start-page: 15 year: 2015 ident: EES_677_4_042031bib25 article-title: Derivation of cell-adapted Sacbrood virus (SBV) from the native Korean honeybee publication-title: Virus Research doi: 10.1016/j.virusres.2014.12.008 contributor: fullname: Kweon – volume: 103 start-page: 13 year: 1983 ident: EES_677_4_042031bib11 article-title: Association of viruses with two protozoal pathogens of the honey bee publication-title: Ann. 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Biol doi: 10.1111/j.1744-7348.1983.tb02735.x contributor: fullname: Bailey – volume: 92 start-page: 152 year: 2006 ident: EES_677_4_042031bib17 article-title: Horizontal and vertical transmission of viruses in the honey bee, Apis mellifera publication-title: Journal of Invertebrate Pathology doi: 10.1016/j.jip.2006.03.010 contributor: fullname: Chen – volume: 10 start-page: 55 ident: EES_677_4_042031bib3 article-title: Sensitivity of a new strain of LPK cells to cattle viruses 2014 publication-title: Veterinary Medicine contributor: fullname: Galnbek – volume: 1 start-page: 31 year: 2017 ident: EES_677_4_042031bib23 article-title: Distribution of RNA-Containing Bee Viruses in a Honey Bee (Apis mellifera) in Certain Regions of Russia publication-title: Molecular Genetics, Microbiology and Virology doi: 10.18821/0208-0613-2017-35-1-31-35 contributor: fullname: Kalashnikov – volume: 7 start-page: 26 year: 2006 ident: EES_677_4_042031bib20 article-title: Viruses of bees publication-title: Beekeeping contributor: fullname: Grobov – volume: 4 start-page: 54 year: 2013 ident: EES_677_4_042031bib7 article-title: The influence of beekeeping products (pollen, bee bread and royal jelly) on the growth of the cell culture of vertebrates and invertebrates publication-title: Veterinary medicine and feeding contributor: fullname: Sayfutdinova – volume: 10 start-page: 274 year: 2011 ident: EES_677_4_042031bib15 article-title: Identification of sacbrood virus disease in honeybee, Apis mellifera L. by using ELISA and RT-PCR technique publication-title: Indian Journal of Byotechnology contributor: fullname: Rana – start-page: 3 year: 2011 ident: EES_677_4_042031bib9 contributor: fullname: Mader Monaghan – start-page: 3 year: 2008 ident: EES_677_4_042031bib8 contributor: fullname: Khangalova – volume: 8 start-page: 1 year: 2013 ident: EES_677_4_042031bib13 article-title: TaqMan MGB Probe Fluorescence Real-Time Quantitative PCR for Rapid Detection of Chinese Sacbrood Virus publication-title: PLOS ONE contributor: fullname: Ma – start-page: 26 year: 2011 ident: EES_677_4_042031bib6 contributor: fullname: Dyakonov – volume: 1 start-page: 72 year: 2012 ident: EES_677_4_042031bib21 article-title: Distribution of RNA-containing viruses in the honey bee Apis mellifera L. on the territory of Russia publication-title: Farm animals contributor: fullname: Kalashnikov – volume: 1 start-page: 1 year: 2013 ident: EES_677_4_042031bib14 article-title: Complete genome sequence of Sacbrood virus strain SBM2, Isolated from the Honeybee Apis cerana in Vietnam publication-title: Genome Announc doi: 10.1128/genomeA.00076-12 contributor: fullname: Nguyen – start-page: 5 year: 1987 ident: EES_677_4_042031bib16 contributor: fullname: Grobov – start-page: 1 year: 2016 ident: EES_677_4_042031bib19 article-title: The high-throughput production of dsRNA against sacbrood virus for use in the honey bee Apis cerana (Hymenoptera: Apidae) contributor: fullname: Zhang – start-page: 7 year: 2009 ident: EES_677_4_042031bib5 contributor: fullname: Dyakonov – volume: 1 start-page: 80 year: 2014 ident: EES_677_4_042031bib22 article-title: Epidemiological state of apiaries during infection of bee colonies with RNA viruses publication-title: Farm animals contributor: fullname: Kalashnikov – volume: 11 start-page: 227 year: 2014 ident: EES_677_4_042031bib10 article-title: Study of Cultural Characteristics and Interference of Peste des Petites Ruminants Virus and Sheep Pox Virus in Co-Culture publication-title: Life Science Journal contributor: fullname: Taranov – volume: 421 year: 2020 ident: EES_677_4_042031bib24 article-title: Epizootic process in quarantine bee diseases publication-title: IOP Conference Series: Earth and Environmental Science contributor: fullname: Sotnikov |
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Snippet | Abstract A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known.... A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a... |
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SubjectTerms | Animal tissues Biotechnology Cell culture Federal budget Infections Kidneys Rounding Sensitivity State budgets Strains (organisms) Viruses |
Title | Study of cell culture strains sensitivity to sacbrood virus |
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