Study of cell culture strains sensitivity to sacbrood virus

• Published in: IOP conference series. Earth and environmental science Vol. 677; no. 4; p. 42031
• Main Authors: Kalinin, A G, Kuleshov, K V, Isaev, Y G
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.03.2021
• Subjects: Animal tissues; Biotechnology; Cell culture; Federal budget; Infections; Kidneys; Rounding; Sensitivity; State budgets; Strains (organisms); Viruses
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/677/4/042031

Abstract A huge number of cell culture strains obtained from one organ (tissue) of an animal, sensitive to causative agents of various infections, are known. However, a comparative characterization of various cell culture strains sensitivity to such bee viruses as SBV has not yet been carried out. This work was carried out in the Department of Cell Biotechnology and the Laboratory of Bee Diseases of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”. For the study, we took cell cultures of the African green monkey kidneys (Vero (3 strains) - Vero (strain from the FSC VIEV Collection)), Vero K (strain from the FSC VIEV Collection), Vero (strain from Germany). We have shown that all 3 of the Vero cell cultures above strains are sensitive to SBV, however, the cell model in which the most pronounced signs of CPD were revealed is the Vero K cell line. The first signs of CPD manifestation virus in the culture of Vero K cells were observed at first passage. After 6 days. screeds were found in the monolayer after infection. At the second passage of the virus after 5 days. culturing CPD manifested itself as the appearance of voids, cell strands, and rounding of the cells part.