Application of spectroscopy ( 1 HMRS) to assess liver metabolite concentrations in rats with intrauterine growth restriction

• Published in: The journal of maternal-fetal & neonatal medicine Vol. 30; no. 8; p. 1001
• Main Authors: Wang, Tao, Chen, Pingyang, Bian, Dujun, Chen, Juncao
• Format: Journal Article
• Language: English
• Published: England 18.04.2017
• Subjects: Animals; Animals, Newborn; Choline - analysis; Choline - metabolism; Creatine - analysis; Creatine - metabolism; Disease Models, Animal; Female; Fetal Growth Retardation - diagnosis; Fetal Growth Retardation - metabolism; Fetal Growth Retardation - pathology; Glucose - analysis; Glucose - metabolism; Glycogen - analysis; Glycogen - metabolism; Lipid Metabolism; Lipids - analysis; Liver - chemistry; Liver - diagnostic imaging; Liver - metabolism; Pregnancy; Proton Magnetic Resonance Spectroscopy; Rats; Rats, Sprague-Dawley; Water - analysis; Water - metabolism; liver metabolism; proton magnetic resonance spectroscopy; Intrauterine growth retardation
• ISSN: 1476-4954
• DOI: 10.1080/14767058.2016.1174989

Proton magnetic resonance spectroscopy ( H-MRS) measurement of liver metabolism in intrauterine growth restriction rats has seldom been reported. This study investigated the application of H-MRS in assessing liver metabolism in newborn pups that experienced intrauterine growth restriction. Intra-uterine growth restriction was established by feeding rats low-protein diets during pregnancy. Newborn pups received conventional magnetic resonance imaging and H-MRS using a 3.0T whole body MR scanner at 3, 8 and 12 weeks post birth. The success rate of H-MRS was 83.33%. Significantly lower body weight, BMI and body length at 3 weeks as well as significantly lower body weight, BMI and waist circumference at 8 and 12 weeks were observed in newborn pups of IUGR rats compared with pups of control rats. Significant differences in ACho/H O, ACr/H O, AGlx/H O and ALipid/H O at 3 and 8 weeks as well as significant differences in ACr/H O, ALipid/H O and AGlx/H O at 12 weeks were observed between pups of control rats and pups of IUGR rats. H-MRS allows noninvasive assessment of liver metabolism in the rat and demonstrated the poor liver development of rats that experienced IUGR.