Stress response in Cu2+ and Cd2+ exposed oysters (Crassostrea gigas): an immunohistochemical approach

Localization of heat shock proteins (HSPs) and metallothioneins (MTs) was investigated in a marine bivalve (Crassostrea gigas) by immunohistochemical methods. Differential protein expression was demonstrated in digestive gland, gonad and gills, using a polyclonal antibody against C. gigas proteins....

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Published inComparative biochemistry and physiology. Toxicology & pharmacology Vol. 141; no. 2; pp. 151 - 156
Main Authors Moraga, Dario, Meistertzheim, Anne-Leïla, Tanguy-Royer, Séverine, Boutet, Isabelle, Tanguy, Arnaud, Donval, Anne
Format Journal Article
LanguageEnglish
Published United States 01.06.2005
Subjects
Animals
Cadmium - toxicity
Copper - toxicity
Enzyme-Linked Immunosorbent Assay
HSP70 Heat-Shock Proteins - analysis
Immunohistochemistry
Metallothionein - analysis
Ostreidae - drug effects
Ostreidae - metabolism
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Summary:Localization of heat shock proteins (HSPs) and metallothioneins (MTs) was investigated in a marine bivalve (Crassostrea gigas) by immunohistochemical methods. Differential protein expression was demonstrated in digestive gland, gonad and gills, using a polyclonal antibody against C. gigas proteins. Application of this technique showed the cellular and tissue immunolabelling specificity of the two proteins. HSPs and MTs were localized in the epithelium of the digestive gland and gills in contact with the palleal compartment. For the first time, localization of MTs was observed in mature gametes of bivalve molluscs. Our results establish a basis for the use of immmunodetection techniques to study the tissue-specific localization of stress proteins in marine bivalves exposed to metal stress.
ISSN:1532-0456
1878-1659
DOI:10.1016/j.cca.2005.05.014