pK a Calculations for class A β‐lactamases: Influence of substrate binding

• Published in: Protein science Vol. 8; no. 2; pp. 404 - 409
• Main Authors: Lamotte‐Brasseur, Josette, Lounnas, Valère, Raquet, Xavier, Wade, Rebecca C.
• Format: Journal Article
• Language: English
• Published: 1999
• ISSN: 0961-8368; 1469-896X
• DOI: 10.1110/ps.8.2.404

Abstract β‐Lactamases are responsible for bacterial resistance to β‐lactams and are thus of major clinical importance. However, the identity of the general base involved in their mechanism of action is still unclear. Two candidate residues, Glu166 and Lys73, have been proposed to fulfill this role. Previous studies support the proposal that Glu166 acts during the deacylation, but there is no consensus on the possible role of this residue in the acylation step. Recent experimental data and theoretical considerations indicate that Lys73 is protonated in the free β‐lactamases, showing that this residue is unlikely to act as a proton abstractor. On the other hand, it has been proposed that the p K a of Lys73 would be dramatically reduced upon substrate binding and would thus be able to act as a base. To check this hypothesis, we performed continuum electrostatic calculations for five wild‐type and three β‐lactamase mutants to estimate the p K a of Lys73 in the presence of substrates, both in the Henri–Michaelis complex and in the tetrahedral intermediate. In all cases, the p K a of Lys73 was computed to be above 10, showing that it is unlikely to act as a proton abstractor, even when a β‐lactam substrate is bound in the enzyme active site. The p K a of Lys234 is also raised in the tetrahedral intermediate, thus confirming a probable role of this residue in the stabilization of the tetrahedral intermediate. The influence of the β‐lactam carboxylate on the p K a values of the active‐site lysines is also discussed.