Large-scale purification and kinetic properties of recombinant hormone-sensitive lipase from baculovirus-insect cell systems

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Bibliographic Details
Published inMethods in enzymology Vol. 284; p. 272
Main Authors Holm, C, Contreras, J A, Verger, R, Schotz, M C
Format Journal Article
LanguageEnglish
Published United States 1997
Subjects
Animals
Baculoviridae
Cell Line
Chromatography, Ion Exchange
Enzyme Stability
Humans
Indicators and Reagents
Kinetics
Rats
Recombinant Proteins - chemistry
Recombinant Proteins - isolation & purification
Recombinant Proteins - metabolism
Spodoptera
Sterol Esterase - chemistry
Sterol Esterase - isolation & purification
Sterol Esterase - metabolism
Substrate Specificity
Transfection - methods
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ISSN:0076-6879
DOI:10.1016/S0076-6879(97)84018-9