Modified polynucleotides.III. De novo synthesis and hydrolysis of poly d(A-r5U) copolymers

• Published in: Nucleic acids research Vol. 1; no. suppl-1; pp. s155 - s160
• Main Authors: Sági, J., Szemzó, A., Ötvös, L.
• Format: Journal Article
• Language: English
• Published: Oxford University Press 1978
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/1.suppl_1.s155

Kinetics of DNA polymerase I enzyme catalyzed de novo synthesis and hydrolysis of poly d(A-r5U) copolymers, where r=H, methyl, ethyl, n-propyl, n-butyl and n-pentyl groups, was studied. Relative rates of polymerization were different from the corresponding rates measured with poly d(A-T) template-primer, which may be the effect of the modified templates. The rate of degradation of the polymers by the exonuclease of DNA polymerase I enzyme depends on the substitution of polynucleotides: with the increase of chain length of r in the polymer, the rate of hydrolysis decreases.