Fibroblasts as the subject of proliferative activity research in vitro

This review presents the data devoted to anatomical and functional diversity of fibroblasts, peculiarities of metabolic processes and energy exchange in these cells. In particular, the changes in fibroblast proliferative activity depending on various factors are discussed. The review shows the influ...

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Published inНаука и инновации в медицине Vol. 5; no. 3; pp. 210 - 215
Main Authors Valeriya I. Kuzmicheva, Larisa T. Volova, Frida N. Gilmiyarova, Ilya M. Bykov, Elena V. Avdeeva, Natalya A. Kolotieva
Format Journal Article
LanguageEnglish
Published Samara State Medical University 20.10.2020
Subjects
cell culture
fibroblasts
lactate
malate dehydrogenase
metabolism
pyruvate
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Summary:This review presents the data devoted to anatomical and functional diversity of fibroblasts, peculiarities of metabolic processes and energy exchange in these cells. In particular, the changes in fibroblast proliferative activity depending on various factors are discussed. The review shows the influence of the malate dehydrogenase shuttle system on the activity of metabolic processes and the life span of fibroblastsin vitro. The increase of cell cultivation timein vitrois associated with the cytosolic isoform of this enzyme. The stability of fibroblast cell culture to the activation of free-radical processes and peroxidation with addition of biologically active compounds is described and followed by a discussion of the role of separate metabolites in providing free-radical protection and maintenance of the proliferative potential of cells.
ISSN:2500-1388
2618-754X
DOI:10.35693/2500-1388-2020-5-3-210-215