The isoprostane, 8-epi-PGF2α, is accumulated in coronary arteries isolated from patients with coronary heart disease
In the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart d...
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Published in | Cardiovascular research Vol. 43; no. 2; pp. 492 - 499 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Oxford University Press
01.08.1999
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Abstract | In the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n = 23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n = 19) or from healthy heart donors (controls, n = 6).
Sections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2 alpha in the vascular intima and media. In addition, the 8-epi-PGF2 alpha content was determined using a specific immunoassay after extraction and purification.
The immunohistochemical results indicated that 8-epi-PGF2 alpha is significantly enriched in arteries from patients suffering from CHD as compared to CMP (P < 0.0001). In controls, significantly less immunostaining was observed. Furthermore, a significant positive correlation between semiquantitative immunohistochemistry and radioimmunological determination was observed too.
From our findings we conclude that 8-epi-PGF2 alpha is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis. |
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AbstractList | OBJECTIVEIn the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n = 23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n = 19) or from healthy heart donors (controls, n = 6).METHODSSections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2 alpha in the vascular intima and media. In addition, the 8-epi-PGF2 alpha content was determined using a specific immunoassay after extraction and purification.RESULTSThe immunohistochemical results indicated that 8-epi-PGF2 alpha is significantly enriched in arteries from patients suffering from CHD as compared to CMP (P < 0.0001). In controls, significantly less immunostaining was observed. Furthermore, a significant positive correlation between semiquantitative immunohistochemistry and radioimmunological determination was observed too.CONCLUSIONSFrom our findings we conclude that 8-epi-PGF2 alpha is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis. In the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n = 23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n = 19) or from healthy heart donors (controls, n = 6). Sections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2 alpha in the vascular intima and media. In addition, the 8-epi-PGF2 alpha content was determined using a specific immunoassay after extraction and purification. The immunohistochemical results indicated that 8-epi-PGF2 alpha is significantly enriched in arteries from patients suffering from CHD as compared to CMP (P < 0.0001). In controls, significantly less immunostaining was observed. Furthermore, a significant positive correlation between semiquantitative immunohistochemistry and radioimmunological determination was observed too. From our findings we conclude that 8-epi-PGF2 alpha is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis. |
Author | EKMEKCIOGLU, C ULLRICH, R TAMADDON, F SINZINGER, H TATZBER, F MORGAN, A OGUOGHO, A MOHAMMAD REZA MEHRABI GRIMM, M GLOGAR, H. D |
Author_xml | – sequence: 1 surname: MOHAMMAD REZA MEHRABI fullname: MOHAMMAD REZA MEHRABI organization: Department of Cardiology, University of Vienna, Wahringer Gurtel 18-20, 1090 Vienna, Austria – sequence: 2 givenname: C surname: EKMEKCIOGLU fullname: EKMEKCIOGLU, C organization: Department of Medical Physiology, University of Vienna, Vienna, Austria – sequence: 3 givenname: F surname: TATZBER fullname: TATZBER, F organization: Department of Nuclear Medicine, University of Vienna, Vienna, Austria – sequence: 4 givenname: A surname: OGUOGHO fullname: OGUOGHO, A organization: Department of Nuclear Medicine, University of Vienna, Vienna, Austria – sequence: 5 givenname: R surname: ULLRICH fullname: ULLRICH, R organization: Department of Clinical Pathology, University of Vienna, Vienna, Austria – sequence: 6 givenname: A surname: MORGAN fullname: MORGAN, A organization: Department of Cardiology, University of Vienna, Wahringer Gurtel 18-20, 1090 Vienna, Austria – sequence: 7 givenname: F surname: TAMADDON fullname: TAMADDON, F organization: Department of Cardiology, University of Vienna, Wahringer Gurtel 18-20, 1090 Vienna, Austria – sequence: 8 givenname: M surname: GRIMM fullname: GRIMM, M organization: Department of Surgery, University of Vienna, Vienna, Austria – sequence: 9 givenname: H. D surname: GLOGAR fullname: GLOGAR, H. D organization: Department of Cardiology, University of Vienna, Wahringer Gurtel 18-20, 1090 Vienna, Austria – sequence: 10 givenname: H surname: SINZINGER fullname: SINZINGER, H organization: Department of Nuclear Medicine, University of Vienna, Vienna, Austria |
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Snippet | In the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of... OBJECTIVEIn the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated... |
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SubjectTerms | Analysis of Variance Biological and medical sciences Cardiology. Vascular system Cardiomyopathy, Dilated - metabolism Coronary Disease - metabolism Coronary Disease - surgery Coronary heart disease Coronary Vessels - chemistry Heart Heart Transplantation Humans Immunohistochemistry Medical sciences Middle Aged Radioimmunoassay |
Title | The isoprostane, 8-epi-PGF2α, is accumulated in coronary arteries isolated from patients with coronary heart disease |
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