The isoprostane, 8-epi-PGF2α, is accumulated in coronary arteries isolated from patients with coronary heart disease

• Published in: Cardiovascular research Vol. 43; no. 2; pp. 492 - 499
• Main Authors: MOHAMMAD REZA MEHRABI, EKMEKCIOGLU, C, TATZBER, F, OGUOGHO, A, ULLRICH, R, MORGAN, A, TAMADDON, F, GRIMM, M, GLOGAR, H. D, SINZINGER, H
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.08.1999
• Subjects: Analysis of Variance; Biological and medical sciences; Cardiology. Vascular system; Cardiomyopathy, Dilated - metabolism; Coronary Disease - metabolism; Coronary Disease - surgery; Coronary heart disease; Coronary Vessels - chemistry; Heart; Heart Transplantation; Humans; Immunohistochemistry; Medical sciences; Middle Aged; Radioimmunoassay; Vascular disease; Human; Immunohistochemistry; Pathology; Prostaglandin; Pathogenesis; Atherosclerosis; Cardiovascular disease; Coronary heart disease; Free radical
• ISSN: 0008-6363; 1755-3245
• DOI: 10.1016/S0008-6363(99)00108-X

In the present study we wanted to know whether 8-epi-PGF2 alpha, which belongs to the class of isoprostanes formed by free radical-mediated peroxidation of arachidonic acid and arachidonyl-containing phospholipids, is enriched in isolated coronary arteries of patients suffering from coronary heart disease (CHD, n = 23) who received allograft heart transplants as compared to vessels derived from patients with dilative cardiomyopathy (CMP, n = 19) or from healthy heart donors (controls, n = 6). Sections from the isolated coronary arteries were analysed by semiquantitative immunohistochemistry by determining the area and intensity of positive reaction for 8-epi-PGF2 alpha in the vascular intima and media. In addition, the 8-epi-PGF2 alpha content was determined using a specific immunoassay after extraction and purification. The immunohistochemical results indicated that 8-epi-PGF2 alpha is significantly enriched in arteries from patients suffering from CHD as compared to CMP (P < 0.0001). In controls, significantly less immunostaining was observed. Furthermore, a significant positive correlation between semiquantitative immunohistochemistry and radioimmunological determination was observed too. From our findings we conclude that 8-epi-PGF2 alpha is especially accumulated in coronary arteries from CHD patients and therefore is likely to be involved in atherogenesis.