Screening test for alpha 1-antitrypsin in dried-blood specimens

We describe a fluorescent spot test for detecting alpha 1-antitrypsin activity in dried-blood specimens. The eluate of a blood disc is mixed with carbobenzoxy-L-arginine-7-amino-4-methylcoumarin amide and trypsin at the appropriate pH. In the absence of alpha 1-antitrypsin, aminomethylcoumarin, a st...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 28; no. 4 Pt 1; pp. 615 - 617
Main Authors Orfanos, A P, Naylor, E W, Guthrie, R
Format Journal Article
LanguageEnglish
Published England 01.04.1982
Subjects
Adult
alpha 1-Antitrypsin - analysis
alpha 1-Antitrypsin - genetics
alpha 1-Antitrypsin Deficiency
Child
Humans
Infant, Newborn
Liver Diseases - blood
Liver Diseases - genetics
Methods
Phenotype
Pulmonary Emphysema - blood
Pulmonary Emphysema - genetics
Risk
Smoking
Specimen Handling
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Summary:We describe a fluorescent spot test for detecting alpha 1-antitrypsin activity in dried-blood specimens. The eluate of a blood disc is mixed with carbobenzoxy-L-arginine-7-amino-4-methylcoumarin amide and trypsin at the appropriate pH. In the absence of alpha 1-antitrypsin, aminomethylcoumarin, a strongly fluorescent compound, is released. The reaction mixture, when spotted on chromatography paper and viewed under ultraviolet light, exhibits a bright fluorescence only in the case of specimens with alpha 1-antitrypsin deficiency. alpha 1-Antitrypsin activity so estimated correlated well with quantitative assays of dried-blood spots and serum. The procedure is simple and inexpensive, and has the potential for use as a screening test.
Bibliography:ObjectType-Article-1
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ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/28.4.615