Approaches in extracellular matrix engineering for determination of adhesion molecule mediated single cell function

The native extracellular matrix (ECM) and the cells that comprise human tissues are together engaged in a complex relationship; cells alter the composition and structure of the ECM to regulate the material and biologic properties of the surrounding environment while the composition and structure of...

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Bibliographic Details
Published inFrontiers in biology Vol. 8; no. 1; pp. 32 - 49
Main Authors Ayres-Sander, Chantal E, Gonzalez, Anjelica L
Format Journal Article
LanguageEnglish
Published Heidelberg Springer-Verlag 01.02.2013
SP Higher Education Press
Subjects
adhesion
bioactive properties
Biochemistry
Biomedical and Life Sciences
cadherins
cell adhesion
Cell Biology
cell movement
cultured cells
Developmental Biology
extracellular matrix
Genetics and Population Dynamics
humans
integrins
Life Sciences
Neurobiology
phagocytosis
Proteomics
Review
tissue repair
natural polymers
Extracellular matrix
integrins
biomaterials
RGD
peptide sequences
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Summary:The native extracellular matrix (ECM) and the cells that comprise human tissues are together engaged in a complex relationship; cells alter the composition and structure of the ECM to regulate the material and biologic properties of the surrounding environment while the composition and structure of the ECM modulates cellular processes that maintain healthy tissue and repair diseased tissue. This reciprocal relationship occurs via cell adhesion molecules (CAMs) such as integrins, selectins, cadherins and IgSF adhesion molecules. To study these cell-ECM interactions, researchers use two-dimensional substrates or three-dimensional matrices composed of native proteins or bioactive peptide sequences to study single cell function. While two-dimensional substrates provide valuable information about cell-ECM interactions, three-dimensional matrices more closely mimic the native ECM; cells cultured in three-dimensional matrices have demonstrated greater cell movement and increased integrin expression when compared to cells cultured on two-dimensional substrates. In this article we review a number of cellular processes (adhesion, motility, phagocytosis, differentiation and survival) and examine the cell adhesion molecules and ECM proteins (or bioactive peptide sequences) that mediate cell functionality.
Bibliography:http://dx.doi.org/10.1007/s11515-012-1199-x
ISSN:1674-7984
1674-7992
DOI:10.1007/s11515-012-1199-x