Effects of rHu-EPO on Myocyte Apoptosis and Cardiac Function Following Acute Myocardial Infarction in Rats

• Published in: Current medical science Vol. 25; no. 1; pp. 55 - 58
• Main Author: 叶亮 杜心灵 夏家红 江平
• Format: Journal Article
• Language: English
• Published: China 2005
• Subjects: Animals; Apoptosis - drug effects; Erythropoietin - pharmacology; Erythropoietin - therapeutic use; Male; Myocardial Infarction - drug therapy; Myocardial Infarction - pathology; Myocardial Infarction - physiopathology; Myocytes, Cardiac - pathology; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Proto-Oncogene Proteins c-bcl-2 - genetics; Rats; Rats, Sprague-Dawley; Recombinant Proteins; rHu-EPO; Ventricular Function - drug effects; 动物模型; 心功能; 急性心肌梗死; 细胞凋亡; 肌细胞
• ISSN: 1672-0733; 2096-5230; 1993-1352; 2523-899X
• DOI: 10.1007/BF02831387

The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery, rHu EPO was administered i.p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2, bax) and TUNEL dyeing. The results showed that hemodynamic function in MI.4- EPO group was much better than in MI group.The number of the cells positive for bax and TUNEL in MI+ EPO group was less than that in MI group. The number of the cells positive for bcl-2 in MI+EPO group was more than that in MIgroup. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.