Photoswitchable hemithioindigo inspired copper ion selective sensing with excellent selectivity and versatile operational modes
Copper is an essential element in living organisms and the monitoring of copper ions in aquatic environments and biological systems is of great significance. Inspired by a family of visible light responsive photoswitchable compounds, we report here a fluorescent probe (HTI-Q) for Cu2+ with excellent...
Saved in:
Published in | Sensors and actuators. B, Chemical Vol. 381; p. 133437 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
15.04.2023
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Copper is an essential element in living organisms and the monitoring of copper ions in aquatic environments and biological systems is of great significance. Inspired by a family of visible light responsive photoswitchable compounds, we report here a fluorescent probe (HTI-Q) for Cu2+ with excellent selectivity, high sensitivity, and a limit of detection (LOD) of ca. 0.02 μM. While the central carbon-carbon double bond of HTI-Q is photoisomerizable between the Z and E configurations, it is locked upon binding with Cu2+ into the E configuration, resulting in a large bathochromic shift (102 nm) and ratiometric fluorescence changes. Different sensing modes were demonstrated including in mixed solvents, a two-phase sensing system containing cation exchanger, and fluorescent nanoprobes containing a reference dye. The absorbance change of HTI-Q upon 470 nm light illumination also depended on the concentration of Cu2+. HTI-Q-based sensors were successfully applied to determine Cu2+ in real water samples with excellent recovery. Further, fluorescent nanoprobes incorporating HTI-Q were successfully applied to image endolysosomal Cu2+ changes upon external stimulation.
[Display omitted]
•A photoswitchable Cu2+ probe (HTI-Q) was proposed for the first time based on hemithioindigo.•HTI-Q offered tunable sensitivity, high selectivity, and several different optical sensing modes.•Nanoprobes containing HTI-Q were successfully applied for subcellular fluorescence imaging in the endolysosomes. |
---|---|
ISSN: | 0925-4005 1873-3077 |
DOI: | 10.1016/j.snb.2023.133437 |