Study on chemical constituents of different species of Dendrobium
The objective of this study is to determine the erianin of 10 species of Dendrobium by high performance liquid chromatography (HPLC). Processing techniques were washed, dried and ground. The research method for determining the content of erianin was adopted from Chinese Pharmacopoeia 2015 Edition. A...
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| Published in | E3S web of conferences Vol. 165; p. 5026 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
EDP Sciences
01.01.2020
|
| Online Access | Get full text |
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| Summary: | The objective of this study is to determine the erianin of 10 species of
Dendrobium
by high performance liquid chromatography (HPLC). Processing techniques were washed, dried and ground. The research method for determining the content of erianin was adopted from
Chinese Pharmacopoeia
2015 Edition. Acetonitrile-0.05% phosphoric acid (37:63) was used as mobile phase. The samples were separated on Sharpsil-TC18 column(4.6*150mm;5μm) at a flow rate of 1.2mL/min and detected at 230nm, and the column temperature was kept at 30℃. The injection volume was 20μL. The number of theoretical plates was not less than 6000 according to the chromatographic peak of erianin. The result of the study showed erianin was detected only in
Dendrobium chrysotoxum
among the 10 species of
Dendrobium
. and the content was 0.098%. Conclusion: The content of erianin in
Dendrobium chrysotoxum
met the specification of no less than 0.03% in the 2015 edition of
Chinese Pharmacopoeia
. |
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| ISSN: | 2267-1242 2267-1242 |
| DOI: | 10.1051/e3sconf/202016505026 |