N-myc amplification at chromosome band 1p32 in neuroblastoma cells as investigated by in situ hybridization

Chromosome deletion at the short arm of one chromosome 1 (1p32)--the most common aberration in neuroblastoma cells--was found to be combined with the generation of a homogeneously staining region at this specific site in a newly established neuroblastoma cell line (GI-LI-N) from a stage IV neuroblas...

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Bibliographic Details
Published inJournal of cancer research and clinical oncology Vol. 114; no. 6; p. 636
Main Authors Longo, L, Christiansen, H, Christiansen, N M, Cornaglia-Ferraris, P, Lampert, F
Format Journal Article
LanguageEnglish
Published Germany 1988
Subjects
Child, Preschool
Chromosome Aberrations
Chromosomes, Human, Pair 1
Female
Gene Amplification
Humans
Infant
Male
Neuroblastoma - genetics
Nucleic Acid Hybridization
Proto-Oncogenes
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Summary:Chromosome deletion at the short arm of one chromosome 1 (1p32)--the most common aberration in neuroblastoma cells--was found to be combined with the generation of a homogeneously staining region at this specific site in a newly established neuroblastoma cell line (GI-LI-N) from a stage IV neuroblastoma. By in situ hybridization this homogeneously staining region was shown to contain multiple copies of the proto-oncogene N-myc. This 30-fold oncogene amplification was confirmed by Southern-blot and DNA-dot-blot analyses. In two additional cell lines from children with stage IV neuroblastoma (GI-ME-N and GI-CA-N) N-myc amplification was not detected. Chromosome 1, however, was involved in a structural rearrangement in one cell line (GI-ME-N).
ISSN:0171-5216
DOI:10.1007/BF00398190