Pattern and value of Sperm DNA Fragmentation Index and its correlation with spermiogram in infertile South West Nigerian Men

• Published in: Morphologie Vol. 108; no. 361; p. 100763
• Main Authors: Ibitoye, B.O., Bashir, F.O., Ibitoye, F.O., Alabi, O., Olaniyan, O.T., Faduola, P., Bamisi, O.D., Ajibare, A.J., Omoseeye, S.D.
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.06.2024
• Subjects: Adult; Cross-Sectional Studies; DNA Fragmentation; Humans; Infertility; Infertility, Male - diagnosis; Infertility, Male - genetics; Infertility, Male - pathology; Male; Middle Aged; Nigeria; Semen Analysis; Sperm chromatin structure assay; Sperm Count; Sperm Motility; Spermatozoa; Spermiogram; Nigeria; Sperm chromatin structure assay; Infertility; Spermiogram; DNA fragmentation
• ISSN: 1286-0115
• DOI: 10.1016/j.morpho.2024.100763

•The mean sperm DFI values is 15.78±8.52. In the studied population.•The DFI is negatively correlated with age, abnormal morphology, motility and concentration.•There is significant correlation between sperm concentration and SDFI.•Age has no significant effect of the value but increase in sperm production does.•Spermiogram cannot fully determine the SDFI value, and there are many factors that determine the value of SDFI aside semen parameters.•On the regression model, abnormal morphology has more impact on the value of SDFI. Semen analysis has been used for a long time to assess male fertility due to its limitations sperm DNA fragmentation index (DFI), which describes the sperm DNA's condition, is an appropriate criterion for assessing male fertility. This study evaluated the pattern and value of DFI of infertile men in the South West of Nigeria. This is a cross-sectional and descriptive study that recruited two hundred and eighty-seven (287) patients from two fertility centers in Lagos, Nigeria. The Sperm DFI was determined using the Sperm chromatin structure assay (SCSA) test. The descriptive and inferential statistics of the study were carried out using R packages (R version 4.2.0) with the help of R functions using compiled code. The result showed that the mean age sperm concentration, total motility morphology, and DFI were as follows 42.96±7.09years, 40.18±4.19×106 per ml, 49%±19%, 56±17%, and 15.78±8.52 respectively. There is a significant negative correlation between sperm concentration and DFI at a P-value of 0.0018 with a regression model of Coefficient of determination is 0.305. The DFI value of infertile men negatively correlates with sperm concentration, thus increase sperm production may improve sperm quality.