Automated imunoanalysis systems for monitoring mammalian cell cultivation processes

Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employ...

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Bibliographic Details
Published inCytotechnology (Dordrecht) Vol. 15; no. 1-3; p. 259
Main Authors Schulze, B, Middendorf, C, Reinecke, M, Scheper, T, Noé, W, Howaldt, M
Format Journal Article
LanguageEnglish
Published United States 1994
Subjects
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - biosynthesis
Automation - instrumentation
Automation - methods
Biotechnology - instrumentation
Biotechnology - methods
Culture Techniques - instrumentation
Culture Techniques - methods
Enzyme-Linked Immunosorbent Assay - methods
Glucose - analysis
Glucose - metabolism
Immunoassay - instrumentation
Immunoassay - methods
Immunoglobulin G - analysis
Immunoglobulin G - biosynthesis
Indicators and Reagents
Mammals
Nephelometry and Turbidimetry - instrumentation
Nephelometry and Turbidimetry - methods
Recombinant Proteins - analysis
Recombinant Proteins - biosynthesis
Time Factors
Tissue Plasminogen Activator - analysis
Tissue Plasminogen Activator - biosynthesis
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Summary:Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
ISSN:0920-9069
DOI:10.1007/BF00762401