Harvey-ras allele deletion detected by in situ hybridization to Wilms' tumor chromosomes

We have examined the chromosomes from a case of sporadic Wilms' tumor using in situ hybridization to determine whether the Ha-ras (c-Ha-ras 1) oncogene had been deleted as the result of a reciprocal chromosomal translocation between the short arm of chromosome 11 (breakpoint 11p13) and the long...

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Bibliographic Details
Published inHuman genetics Vol. 67; no. 2; p. 190
Main Authors Eccles, M R, Millow, L J, Wilkins, R J, Reeve, A E
Format Journal Article
LanguageEnglish
Published Germany 01.07.1984
Subjects
Alleles
Autoradiography
Cells, Cultured
Chromosomes, Human, 6-12 and X
DNA, Neoplasm
Genetic Techniques
Humans
In Vitro Techniques
Kidney Neoplasms - genetics
Nucleic Acid Hybridization
Oncogenes
Polymorphism, Genetic
Translocation, Genetic
Wilms Tumor - genetics
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Summary:We have examined the chromosomes from a case of sporadic Wilms' tumor using in situ hybridization to determine whether the Ha-ras (c-Ha-ras 1) oncogene had been deleted as the result of a reciprocal chromosomal translocation between the short arm of chromosome 11 (breakpoint 11p13) and the long arm of chromosome 12 (breakpoint 12q13). Neither the derivative 11 nor derivative 12 chromosome hybridized significantly to the Ha-ras probe, which indicated that this cellular oncogene was deleted as a consequence of the translocation. This conclusion is supported by a Southern blot analysis which demonstrates loss of a Harvey-ras allele. These results support the view that the Ha-ras oncogene may be functionally involved in Wilms' tumor development.
ISSN:0340-6717
DOI:10.1007/BF00272999