The rat insulin‐degrading enzyme

The primary structure of the rat insulin‐degrading enzyme (IDE) was determined by cDNA analysis. Rat IDE, as well as the previously characterized homologs from human and Drosophila, contain the carboxyl‐terminal consensus sequence A/S‐K‐L for peroxisome targeting. A stretch of 43 bp surrounding an a...

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Bibliographic Details
Published inFEBS letters Vol. 317; no. 3; pp. 250 - 254
Main Authors Baumeister, Hans, Müller, Dieter, Rehbein, Monika, Richter, Dietmar
Format Journal Article
LanguageEnglish
Published 15.02.1993
Subjects
Insulin-degrading enzyme
Peroxisome
Polyadenylation site
Testis
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Summary:The primary structure of the rat insulin‐degrading enzyme (IDE) was determined by cDNA analysis. Rat IDE, as well as the previously characterized homologs from human and Drosophila, contain the carboxyl‐terminal consensus sequence A/S‐K‐L for peroxisome targeting. A stretch of 43 bp surrounding an alternatively used polyadenylation site is highly conserved between rat and human, suggesting that it may contain important regulatory information. Northern blot analysis revealed two IDE transcripts of 3.7 and 5.5 kb in various tissues. Testis was found to be exceptional in having three different RNAs (3.7, 4.1 and 6.1 kb) at a relatively high abundance. The expression of the IDE gene in testis is correlated with sexual maturation.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)81286-9