Deuteromethylactin B from a Freshwater-derived Streptomyces sp

• Published in: Natural product sciences Vol. 21; no. 4; pp. 261 - 267
• Main Authors: Shaikh, Anam F., Elfeki, Maryam, Landolfa, Samantha, Tanouye, Urszula, Green, Stefan J., Murphy, Brian T.
• Format: Journal Article
• Language: English
• Published: 한국생약학회 2015
• Subjects: 약학; Streptomyces; Octalactin; Freshwater; Actinomycete
• ISSN: 1226-3907; 2288-9027
• DOI: 10.20307/nps.2015.21.4.261

Compared to their terrestrial and marine counterparts, little is known about the capacity of freshwater-derived actinomycete bacteria to produce novel secondary metabolites. In the current study, we highlight the disparities that exist between cultivation-independent and -dependent analyses of actinomycete communities from four locations in Lake Michigan sediment. Furthermore, through phylogenetic analysis of strains isolated from these locations, we identified a Streptomyces sp., strain B025, as being distinct from other Streptomyces spp. isolated from sediment. Upon fermentation this strain produced a rare class of eight-membered lactone secondary metabolites, which have been for their antitumor properties. We used spectroscopic and chemical derivitization techniques to characterize octalactin B (1) in addition to its corresponding novel, unnatural degradation product (2). KCI Citation Count: 0