Roundup-induced biochemical and histopathological changes in the liver and kidney of rats: the ameliorative effects of Linum usitatissimum oil

• Published in: Acta biochimica Polonica Vol. 67; no. 1; p. 53
• Main Authors: Djaber, Nesrine, Ounaceur, Lynda Sabrina, Moubine, Baya Nouha, Khaldi, Taha, Rouag, Mereim, Berrouague, Salma, Amara, Hichem, Taibi, Faiza, Boumendjel, Mahieddine, Boumendjel, Amel, Messarah, Mahfoud
• Format: Journal Article
• Language: English
• Published: Poland 18.03.2020
• Subjects: Animals; Antioxidants - pharmacology; Biomarkers - blood; Flax - chemistry; Glycine - analogs & derivatives; Glycine - toxicity; Glyphosate; Kidney - drug effects; Kidney - pathology; Linseed Oil - pharmacology; Linseed Oil - therapeutic use; Liver - drug effects; Liver - pathology; Oxidative Stress - drug effects; Protective Agents - pharmacology; Protective Agents - therapeutic use; Rats
• ISSN: 0001-527X; 1734-154X
• DOI: 10.18388/abp.2020_2898

The present study was undertaken to evaluate the protective effects of Linum usitatissimum oil (LuO) against sub-chronic Roundup (RDP)-induced toxicity and oxidative stress in rats. Rats were divided into four groups: control group (no treatment), RDP group (Roundup at 269.9 mg/kg b.w.), LuO group (0.5 g/kg b.w. of LuO) and RDP+LuO group (RDP and LuO simultaneously). LuO decreased the ferric reducing antioxidant power (FRAP) (IC50=10.36 μg/ml) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) (IC50=22.85 mg/ml) in the tested tissues. The 30-day exposure of rats to RDP caused an increase in serum hepatic and renal markers: AST, ALT, ALP, LDH, γGT, bilirubin, urea, and creatinine. In addition, SOD, CAT and GST activities decreased by 43%, 61%, and 61%, respectively, while GPx activity, MDA and PCOs levels increased by 80%, 46%, 25%, respectively. LuO treatment alleviated hepatotoxicity in RDP-treated rats, showing improved levels of oxidative stress biomarkers and plasma biochemical parameters. The histological examination of the liver and kidney confirmed the changes in Roundup-treated rats and demonstrated the protective role of LuO.