Determination of refractive index for single living cell using integrated biochip

This paper reports a novel method for measuring the effective refractive index (RI) of single living cell with a small integrated chip. This microchip is able to determine the RI of living cell in real time without extra requirements of fluorescence labeling and chemical treatments, offering low cos...

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Bibliographic Details
Published inThe 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05 Vol. 2; pp. 1712 - 1715 Vol. 2
Main Authors Liang, X.J., Liu, A.Q., Zhang, X.M., Yap, P.H., Ayi, T.C., Yoon, H.S.
Format Conference Proceeding
LanguageEnglish
Published IEEE 2005
Subjects
Cancer
Chemicals
Costs
Diode lasers
Diseases
Fluorescence
Labeling
Lenses
Refractive index
Semiconductor device measurement
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Summary:This paper reports a novel method for measuring the effective refractive index (RI) of single living cell with a small integrated chip. This microchip is able to determine the RI of living cell in real time without extra requirements of fluorescence labeling and chemical treatments, offering low cost and high accuracy meanwhile. It might provide an efficient approach for diseases or cancer diagnosis. The measurement system integrates laser diode, microlenses, and microfluidic channels onto a monolithic chip. In the experiments, two standard polystyrene beads with nominal RIs are, employed to calibrate the system and five types of cancerous cells are subsequently measured. The results indicate that the RI of the tested cells ranges from 1.392 to 1.401, which is larger than typical value 1.35-1.37 for normal cells.
ISBN:0780389948
9780780389946
ISSN:2159-547X
DOI:10.1109/SENSOR.2005.1497421