Anti-arthritic activity of solvent extracts of the bulbs of Crinum pedunculatum R.Br

• Published in: Pharmaceutical sciences Asia Vol. 49; no. 6; pp. 526 - 533
• Main Authors: Doe, Peace, Danquah, Cynthia Amaning, Ossei, Paul Poku Sampene, Ohemeng, Kwasi Adomako, Ofori, Michael, Ativui, Selase, Jibira, Yakubu
• Format: Journal Article
• Language: English
• Published: Faculty of Pharmacy, Mahidol University 2022
• Subjects: arthritis; complete freund’s adjuvant; crinum pedunculatum r.br; cytokines; rheumatoid factor
• ISSN: 2586-8195; 2586-8470
• DOI: 10.29090/psa.2022.06.22.138

Crinum pedunculatum (R.Br) has been used by traditional medicine practitioners in Ghana for the topical management of inflammation and associated disorders. This study investigated the anti-arthritic activity of the methanol, ethanol and ethyl acetate extract of the bulbs of Crinum pedunculatum using Sprague Dawley rats. The anti-arthritic activity of Crinum pedunculatum at various doses (100, 200 and 400 mg/kg) was evaluated using Complete Freund’s Adjuvant (CFA) in rat model. After successful induction of the arthritis, the paw oedema, hematological parameters, pro-inflammatory cytokines and arthritis marker were assessed. Also, the radiological and histopathological evaluations were also performed. There was a significant inhibition (P<0.01) of paw oedema after treatment with 100, 200 and 400 mg/kg of the methanol, ethanol and ethyl acetate Crinum pedunculatum extracts relative to the control. There was also a significant increase in haemoglobin and RBC levels as well as a decrease in WBC count. All the extracts of Crinum pedunculatum decreased IL-6 and Rheumatoid factor serum concentrations and improved joint and cartilage destruction in both contralateral and ipsilateral paw of CFA-induced rats. Significant anti-arthritic activity was observed by the 200 and 400 mg/kg ethanol and methanol Crinum pedunculatum extract. This study confirms that the bulb of Crinum pedunculatum has an anti-arthritic potential and the findings justify the folkloric use the plant as an anti-inflammatory agent.