Isolation of compound microsatellite markers for the common Mediterranean shrub Smilax aspera (Smilacaceae)

Premise of the study: Microsatellite markers were developed for the common Mediterranean shrub Smilax aspera to study the population genetics of this species. METHODS: and Results: Using the compound microsatellite marker technique, a total of 14 pairs of primers were developed for S. aspera, of whi...

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Published inAmerican journal of botany Vol. 98; no. 3; pp. e64 - e66
Main Authors Xu, Xi-hui, Wan, Ying, Qi, Zhe-Chen, Qiu, Ying-xiong, Fu, Cheng-Xin
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.03.2011
Botanical Society of America, Inc
Subjects
alleles
Botany
DNA primers
DNA Primers - metabolism
Flowers & plants
genetic markers
Genetic Testing
genetic variation
Genetics
loci
Mediterranean
Mediterranean Region
microevolution
microsatellite repeats
Microsatellite Repeats - genetics
microsatellites
Polymorphism
population genetics
shrubs
Smilax
Smilax - genetics
Smilax aspera
Taxonomy
Greece
Mediterranean region
Italy
Mediterranean Region
Mediterranean Area
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Summary:Premise of the study: Microsatellite markers were developed for the common Mediterranean shrub Smilax aspera to study the population genetics of this species. METHODS: and Results: Using the compound microsatellite marker technique, a total of 14 pairs of primers were developed for S. aspera, of which 11 were polymorphic, and three were monomorphic. Levels of polymorphism in the 11 markers were checked in 43 individuals collected from two populations in Greece and Italy. The number of alleles per locus ranged from 10 to 26, with an average of 15.55 alleles per locus. All of these primers also could be amplified from a second species, S. hispida. CONCLUSIONS: These microsatellite markers can be used for future studies of genetic diversity in S. aspera, as well as in other related taxa, and will help us to improve our understanding of the microevolutionary processes of this species in the Mediterranean region.
Bibliography:This research was supported by the Key Program of the National Science Foundation of China (grant no. 30830011), the National Basic Research Program of China (973 Program, grant no. 2007CB411600), and the Zhejiang Provincial Natural Science Foundation of China (Y507195). The authors thank Yunpeng Zhao for help during fieldwork. We also gratefully thank Kenneth M. Cameron (UW‐Madison) for editing the manuscript.
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ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1000447