Visualization of invisible cell-death sign by electric-double-layer modulation

Leakage and self-spreading of a cytoplasmic lipid droplet in association with cell death, which was totally invisible in a conventional optical microscope, was discovered by using the recently developed electric-double-layer modulation imaging (EDLMI). The method allowed real-time imaging of the lea...

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Published inBiosensors and bioelectronics. X Vol. 14; p. 100390
Main Authors Tsutsumi, Jun'ya, Kurosu, Jun, Kanai, Kaname, Uchida, Kingo, Morishita, Kana, Sumaru, Kimio
Format Journal Article
LanguageEnglish
Published Elsevier 01.09.2023
Subjects
Apoptosis
Bioimaging
Label-free
Live-cell imaging
Necrosis
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Summary:Leakage and self-spreading of a cytoplasmic lipid droplet in association with cell death, which was totally invisible in a conventional optical microscope, was discovered by using the recently developed electric-double-layer modulation imaging (EDLMI). The method allowed real-time imaging of the leaked lipid droplet with the thickness of a few nanometers. We demonstrate that the discovered phenomenon is useful as a new cellular sign for diagnosis of cell death.
ISSN:2590-1370
2590-1370
DOI:10.1016/j.biosx.2023.100390